plant surface microbiology.pdf

13 Root Surface in Ectomycorrhizas 219
a b
cc
sl
hy
cc
hy
c ccw d
walls of the ectomycorrhiza-forming fungus P. tinctorius. Investigation of the
attachment of Laccaria amethystea (Bull.) Murrill to Picea abies short roots
showed formation of an adhesion pad (Fig. 7a) which was strongly stained by
the Swift reaction for cysteine-rich proteins (Lewis and Knight 1977; Kottke
1997). The surface of the hyphae in contact to the suberin layer and to each
other is stained similarly (Fig. 7b). Attachment of hyphae to the basis of root
hairs by Swift-positive material was found previously for P. tinctorius and
cc
ph
sl
cj
hy
ccw
Fig. 7. a Adhesion pad of Laccaria amethystea hyphae in contact with the suberin layer
of a root cap cell on top of the cortical cell. Scale 0.5 mm. b Swift positive reaction of cysteine-rich
proteins in the cell wall of hyphae in contact with the root and each other
(Laccaria amethystea–Picea abies). Scale 1.5 mm. c Long root of Picea abies displaying no
attachment of Laccaria amethystea hyphae. Scale 15 mm. d Immunogold labelling of
methyl-esterified pectins by the monoclonal antibody JIM7. The cell junction is covered
by a suberized root cap cell wall lined by phenolic residues (short root of Picea abies).
Scale 0.5 mm. cc Cortical cell, ccw cortical cell wall, cj cell junction, ph phenolic residues,
hy hypha, sl suberin layer