plant surface microbiology.pdf

30 Axenic Culture of Symbiotic Fungus Piriformospora indica 603
Table 6. Growth of P. indica on unbuffered aspergillus broth medium supplemented
with different carbon sources
Sugars (w/v) Fresh weight Remarks
(g/l)
Sucrose(0.5 %) 148.8 Compact and numerous chlamydospores
Glucose (1 %) 184.8 Loose, peg-like bodies, few chlamydospores
Fructose (1 %) 78.0 Compact, numerous tiny chlamydospores
Glucose + fructose 109.6 Loose, a few chlamydospores, turned slimy
(0.5 % each)
Data represent an average of three replicates; biomass measured after 7 days.
combination of glucose and fructose (each 0.5 % w/v) led to a medium
increase in the biomass of P. indica (Table 6). On supplementation of glucose
by a mixture of glucose, fructose and sucrose (each 0.5 % w/v), the former
was consumed completely and then the sucrose was metabolized by production
of invertase. This led to an increase of the fructose concentration of the
medium. After the complete consumption of free glucose there was a slow
utilization of fructose.
Fig. 9. P. indica colonies produced in aspergillus broth medium fortified with glucose,
sucrose or fructose. An enlarged view of a colony showing protuberances and peg-like
structures on glucose medium