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plant surface microbiology.pdf

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478<br />

A<br />

cuticle<br />

B<br />

Daniel Knoll and Lukas Schreiber<br />

sterilization experimental setup<br />

UV-light<br />

stainless steel<br />

chamber<br />

inoculation washing<br />

C<br />

bacteria<br />

pressurised air<br />

water<br />

reservoir<br />

stainless steel<br />

chamber<br />

metal ring<br />

sampling port<br />

stopper<br />

measurement<br />

lid<br />

filter incubation box metal grid<br />

nutrient<br />

solution<br />

or water<br />

50 µl<br />

petri dish<br />

Fig. 5. Scheme of the experimental set-up for the in vitro study of microorganisms–leaf<br />

cuticle interactions. A Enzymatic isolated cuticular membranes are sterilised by UV<br />

radiation and mounted in a stainless steel chamber. The chamber is filled with nutrient<br />

solution or water. B The physiological outer side of the cuticle is inoculated with a microbial<br />

cell suspension for 6 h at 25 °C. Microbial cells not bound to the cuticle <strong>surface</strong> are<br />

removed by washing the cuticle with deionised water. Samples of the solution inside the<br />

chamber can be taken with a sterile syringe via closable sampling ports. C Inoculated<br />

cuticles are incubated up-side down on a metal grid in sterile incubation boxes at 25 °C.<br />

Pressurised air of the desired moisture level is conducted through the incubation box

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