plant surface microbiology.pdf

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Table 1. Reporter genes commonly used for the detection of bacteria in environmental applications
Gene Gene product or function Advantages and disadvantages for use in the rhizophere References
Thomas F.C. Chin-A-Woeng and Ben J. J. Lugtenberg
gusA b-Glucuronidase No background in rhizobia and plants. Requires substrate. Sharma and Signer (1990);
Streit et al. (1992)
lacZ b-Galactosidase High background in most plants and bacteria. Drahos et al. (1986);
Katupitiya et al. (1992);
Krishnan and Pueppke (1992)
phoA Alkaline phosphatase High background in most plants and bacteria. Reuber et al. (1991)
xylE Catechol 2,3-dioxygenase Soluble end product. Winstanley et al. (1991)
luxA, luc Luciferase Amplification and or photographic exposure for detection. O’Kane et al. (1988);
de Weger et al. (1991);
Low resolution. Silcock et al. (1992);
de Weger et al. (1997)
celB b-Glucosidase Detection after denaturation of endogenous enzymes. Voorhorst et al. (1995)
tfdA 2,4-Dichlorophenoxyacetate Low resolution. King et al. (1991)
monooxygenase
gfp, bfp, yfp, Autofluorescent protein High resolution. Real-time application. Requires oxygen Chalfie et al. (1994)
cfp, rfp for proper folding.
Antibiotic Antibiotic resistance Requires plate counting. Hagedorn (1994)
resistance
Heavy metal Heavy metal resistance Requires plate counting. de Lorenzo (1994)
resistance