plant surface microbiology.pdf

254
Giang Huong Pham et al.
Fig. 13a–d. Interaction with in vitro grown Populus clone Ech5 on WPM. Explants with
a length of 2 cm, including one terminal and 5–6 side buds were transferred to Magenta
GA7 vessels containing 80 ml of WPM medium. a Top view of a noninoculated control
after 4 weeks of cultivation at 25 °C in constant light; b four discs of P. indica culture
(snowflake) were placed onto the medium and incubated for 7 days at 25 °C prior to the
introduction of the plant cuttings; c normal rooting started at the cutting site (arrow)
after 1 week. The photo shows the typical rooting pattern after 4 weeks; d rooting pattern
of an inoculated plantlet after 4 weeks of co-cultivation with P. indica; rooting at the cutting
site was completely blocked (arrow). Instead, aerial rooting appeared above the
medium, ca. 1 cm apart from the cutting site (arrow). Root tips, when in contact with the
medium, showed a modified morphology (see circles). Inset shows a magnified view of
the modified root tips