plant surface microbiology.pdf

30 Axenic Culture of Symbiotic Fungus Piriformospora indica 601
Table 2. The data represent an average of P. indica biomass of 5 replicates grown in 100
ml aspergillus broth medium in 250 ml capacity Erlenmeyer flasks. Incubation was done
on a rotary shaker (GFL 3.19, Germany) at 144 rpm at 30 °C
Days Biomass (g)
Fresh Dry
5 3.67±0.84 0.06±0.02
7 2.99±0.38 0.06±0.01
10 2.39±0.01 0.07±0.01
Fig. 7. Growth of P. indica on aspergillus broth medium under constant shaking condition
at 25 °C for 7 days. Colonies of different developmental stages were shown. Mature
colonies have the appearance of sea urchins
5 7 10
5 7 10
LM A LM A LM A
3.6 ± 0.15 10.2 5.4 ± 0.36 22.9 7.5 ± 0.16 43.9
Fig. 8. A comparative linear growth of P. indica on aspergillus solidified medium. Measurements
were made after 5, 7 and 10 days, respectively. Incubation was conducted in
dark at 25 °C. Parameter selected was the diameter of 5 replicates of the linear measurement
(LM). Readings are given in cm standard deviation and area (A) on agar medium.
Statistical analysis of the data showed P