plant surface microbiology.pdf

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Gopi K. Podila and Luisa Lanfranco
Table 2. Differential expression of selected interaction clones from L. bicolor. Clones are
selected from macroarray analysis. The E ratio was calculated by comparing the expression
levels in the interacted fungal tissue with those in the free-living fungus using
macroarrays
GenBank no. E value Database match Expression
ratio
BI094576 3e –69 BiP protein (Aspergillus nidulans) 4.10
BI094582 2e –69 PF6.2.1 (Laccaria bicolor) 8.00
BI094583 2e –48 a-tubulin (Ustilago maydis) 4.51
BI094587 1e –10 Homeobox genes Hox-2.6 (Mus musculus) 3.30
BI094592 2e –10 PEP carboxykinase (Mus musculus) 4.33
BI094601 2e –63 LbAut7 (L. bicolor) 3.73
BI094606 3e –09 b-importin (Schizosaccharomyces pombe) 4.43
BI094612 2e –35 Malate synthase (L. bicolor) 3.82
BI094615 9e –36 TEF (EF1a) (Schizophyllum commune) 3.61
BI094619 1e –06 IRS 1-like protein (Xenopus laevis) 3.13
BI094621 1e –31 Ras related protein (L. bicolor) 3.81
BI094622 3e –86 AAD (Phanerochate. chrysosporium) 2.61
BI094623 1e –06 LZK protein kinase (Homo sapiens) 2.90
BI094629 7e –13 Lactonohydrolase (Fusarium oxysporum) 3.91
BI094632 9e –27 E-MAP-115 (H. sapiens) 4.21
BI094635 1e –26 SUG1 subunit 8 (S. cerevisiae) 4.61
BI094639 1e –16 Septin Spn3 (S. pombe) 3.92
BI094653 5e –62 Rho GTPase (S. cerevisiae) 3.21
BI094657 1e –10 Clathrin adapter protein (A. thaliana) 2.08
BI094660 6e –21 AcetylCoA acetyltransferase (L. bicolor) 4.25
BI094667 1e –20 b-transducin (S. pombe) 4.11
BI094676 2e –37 Chitin synthase I (U. maydis) 3.71
shown in Fig. 6.An E ratio that indicates the relative increase in the expression
of each gene in the interaction over the free-living state is used to quantitate
differential expression. There is an overall increase in levels of expression of
several clones tested (Fig. 7). The scatter plot of the normalized data from signal
analysis of the membranes is presented in Fig. 8, which shows global
changes in the expression of interaction related genes. Levels of expression of
selected genes from 72-h interaction are listed in Table 2.
12 Conclusions
The EST and macroarray approaches provide efficient tools for mycorrhizal
symbiosis research. These approaches have the resolution and ability to
obtain a more comprehensive view of various stages of mycorrhiza development
or treatment effects due to nutritional changes or differences due to host