plant surface microbiology.pdf

15 Interaction of Piriformospora indica with Diverse Microorganisms and Plants 239
initially blocked the growth of P. indica, but the fungus rapidly recovered.
Bacillus subtilis had neutral effects, whereas strains of Azospirillum and
Bradyrhizobium promoted the growth of the fungus.
2.2 Chlamydomonas reinhardtii
Ch. reinhardtii and P. indica were allowed to grow on MMN 1/10 medium. The
alga was inoculated as a streak either only on one side or on both sides of a
fungal disc. Both microorganisms grew well in both experiments, but the
growth of the alga was more intense and the color of the colony was a much
darker green if there was only one streak (Fig. 2). At this stage, to our knowledge,
no suitable explanation can be offered for these phenomena.
2.3 Sebacina vermifera
One disc each of P. indica and S. vermifera were placed on Aspergillus
medium. The distance between the two inocula was 4 cm. Both fungi grew
normally without inhibiting the growth of each other. The most interesting
part was that after 7 days at the intersection of two colonies, hyphae turned
highly intertwined, inflated and produced a large number of chlamydospores.
Therefore, both strains were able to block each other with a typical deadlock
Fig. 2. Interaction with Chlamydomonas reinhardtii. P. indica was grown on MS
medium for 48 h. Thereafter, the green alga was streaked on one side (left) or on both
sides (right) of the mycelium. Incubation was carried out under 52 µmol/m light and
24±2 °C temperature. Left Dark green strongly grown algal colonies