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Contents XXIII<br />

6.3 Examples of Expressed Sequence Tag Data Analysis . . . . . 579<br />

7 Macroarrays . . . . . . . . . . . . . . . . . . . . . . . . . . . 582<br />

7.1 PCR Amplification of cDNA Inserts . . . . . . . . . . . . . . 582<br />

7.2 Purification and Quantification of PCR Products . . . . . . 583<br />

7.3 Printing of Macroarrays . . . . . . . . . . . . . . . . . . . . 583<br />

7.4 Generation of Exponential cDNA Probes from<br />

RNA for Macroarrays and Hybridization Analysis . . . . . . 584<br />

7.5 Exponential Amplification of the sscDNAs . . . . . . . . . . 585<br />

8 Generation of Radiolabeled Probes . . . . . . . . . . . . . . 585<br />

9 Hybridization of Macroarrays to Radiolabeled Probes . . . 586<br />

10 Data Analysis . . . . . . . . . . . . . . . . . . . . . . . . . . 586<br />

10.1 Data Analysis Autoradiography Images on X-ray Films . . . 587<br />

11 Example of Laccaria bicolor Macroarrays . . . . . . . . . . . 588<br />

12 Conclusions . . . . . . . . . . . . . . . . . . . . . . . . . . . 590<br />

References and Selected Reading . . . . . . . . . . . . . . . . . . . . . 591<br />

30 Axenic Culture of Symbiotic Fungus Piriformospora indica 593<br />

Giang Huong Pham, Rina Kumari, Anjana Singh,<br />

Rajani Malla, Ram Prasad, Minu Sachdev,<br />

Michael Kaldorf, Francois Buscot, Ralf Oelmuller,<br />

Rüdiger Hampp, Anil Kumar Saxena, Karl-Heinz Rexer,<br />

Gerhard Kost and Ajit Varma<br />

1 Introduction . . . . . . . . . . . . . . . . . . . . . . . . . . . 593<br />

2 Morphology . . . . . . . . . . . . . . . . . . . . . . . . . . . 593<br />

3 Taxonomy of the Fungus . . . . . . . . . . . . . . . . . . . . 595<br />

4 Chlamydospore Formation and Germination . . . . . . . . 597<br />

5 Cultivation . . . . . . . . . . . . . . . . . . . . . . . . . . . . 597<br />

6 Carbon and Energy Sources . . . . . . . . . . . . . . . . . . 600<br />

7 Biomass on Individual Amino Acids . . . . . . . . . . . . . . 604<br />

8 Growth on Complex Media . . . . . . . . . . . . . . . . . . . 604<br />

9 Phosphatic Nutrients . . . . . . . . . . . . . . . . . . . . . . 605<br />

10 Composition of Media . . . . . . . . . . . . . . . . . . . . . 606<br />

11 Conclusions . . . . . . . . . . . . . . . . . . . . . . . . . . . 612<br />

References and Selected Reading . . . . . . . . . . . . . . . . . . . . . 612<br />

Subject Index . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 615

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