plant surface microbiology.pdf

29 Functional Genomic Approaches for Studies of Mycorrhizal Symbiosis 591
responses. In addition, since they require a relatively modest budget, compared
to genome sequencing or microarray-based methods, they are easily
accessible for many academic research groups. With increased use of these
techniques using a variety of mycorrhizal symbiosis models, data can be
exchanged and compared between different laboratories and eventually will
provide a platform to understand the key players (genes) that are markers for
ectomycorrhizal or AM fungal symbioses. In the last couple of years, several
laboratories have begun using these approaches to unravel the mycorrhizal
symbiosis (Martin et al. 2001; Voiblet et al. 2001; Podila et al. 2002; Polidori et
al. 2002).
References and Selected Reading
Adams MD, Kelley JM, Gocayne JD, Dubnick M, Polymeropoulos M, Xiao H, Merril C,
Wu A, Olde B, Moreno R (1991) Complementary DNA sequencing, expressed
sequence tags and humane genome project. Science 252:1651–1656
Altschul SF, Madden TL, Schaffer AA, Zhang J, Zhang Z, Miller W, Lipman DJ (1997)
Gapped Blast and PSI-BLAST: a new generation of protein database search programs.
Nucl Acids Res 25:3389–3402
Bertucci F, Bernard K, Loriod B, Chang YC, Granjeaud S, Birnbaum D, Nguyen C, Peck K,
Jordan BR. (1999) Sensitivity issues in DNA array-based expression measurements
and performance of Nylon microarrays for small samples. Hum Mol Genet 8(9):1715–
22
Bianciotto V, Bonfante P (1992) Quantification of the nuclear content of two arbuscular
mycorrhizal fungi. Mycol Res 96:1071–1076
Chomoczynski P, Sacchi N (1987) Single-step method of RNA isolation by acid guanidinium
thiocyanate-phenol-chloroform extraction. Anal Biochem 162:156–159
Corpet F (1988) Multiple sequence alignment with hierarchical clustering. Nucleic Acids
Res 16:10881–10890
Doudrick RL, Raffle VL, NelsonCD, Fournier GR (1995) Genetic analysis of homokaryons
from a basidiome of Laccaria bicolor using random amplified polymorphic
DNA (RAPD) markers. Mycol Res 99:1361–1365
Eisen MB, Spellman PT, Brown PO, Botstein D (1998) Cluster analysis and display of
genome-wide expression patterns. Proc Natl Acad Sci USA 95:14863–14868
Gianinazzi-Pearson V, van Tuinen D, Dumas-Gaudot E, Dulieu H (2001) Exploring the
genome of Glomalean fungi. In: Hock B (ed) The Mycota, vol IX. Fungal Associations.
Springer, Berlin Heidelberg New York, pp 3–17
Gonzalez P, Zigler S, Epstein DL, Borras T (1998) Identification and isolation of differentially
expressed genes from very small tissue samples. Biotechniques 26:884–892
Graham JH (2000) Assessing costs of arbuscular mycorrhizal symbiosis in agroecosystems.
In: Podila GK, Douds DD (eds) Current advances in mycorrhizae research. APS
Press, St. Paul, MN, pp 127–140
Graham JH, Eissenstat DM (1998) Field evidence for carbon costs of citrus mycorrhizas.
New Phytol 140:103–110
Harrison MJ (1999) Molecular and cellular aspects of the arbuscular mycorrhizal symbiosis.
Ann Rev Plant Physiol Plant Mol Biol 50:361–389
Hofte H, Desprez T, Amselem J, et al. (1993) An inventory of 1152 expressed sequence
tags obtained by partial sequencing of cDNA from Arabidopsis thaliana. Plant J
4:1051–1061