Neue Wege in der Diagnostik und Therapie bei dystrophischen

Anlage 123
6 Anlage
Exon Primer forward Primer reverse
Tabelle 1a
Primer und PCR-Bedingungen beim BIGH3-Gen
Annealing-
Temperatur (°C)
Konzentration
MgCl 2 (mM)
Ex1 5`-GCG CTC TCA CTT CCC TGG AG-3` 5`-GAC TAC CTG ACC TTC CGC AG-3` 60 1,5
Ex2 5`-TAG TAT TTT AGG GCT TCA ATC-3` 5`-AGG CAG GAC AGT AGG ACA GA-3` 58 1,5
Ex3 5`-TTC ACC CAC CAT TCC TCT TC-3` 5´-GGT ACT CCT CTC TCC CAC CA-3` 58 1,5
Ex4 5`-CTC GTC CTC TCC ACC TGT AG-3` 5`-ACT GCA GAC TCC CAT TCA TC-3` 58 1,5
Ex5 5`-TGT TTC CCA GAG TTG CAA G-3` 5`-GAA ACC AGC CCA CAC ATG-3` 50 2
Ex6 5`-TTT GGG ACT ATG CCT CTG-3` 5`-ATG GAG AAT TTC ACA GGT TAG-3` 51 3
Ex7 5`-AAG TGT GCC AAG TTG ACC TC-3` 5`-GGC AGG TGG TAT GTT CAT CT-3` 54 2
Ex8 5`-AGA AGG CGA GGA GGA TCT GG- 5`-CAG TGG CCG AGA AGC TGT GA-3`
3`
60 1,5
Ex9 5`-ACA TTC CTG CTG ATG TGT G-3` 5`-GTT GAG CTG AGT GGA AGT TC-3` 54 2
Ex10 5`-TCT GGA CCT AAC CAT CAC CC-3` 5`-CAG GAG CAT GAT TTA GGA CC-3` 54 1,5
Ex11 5`-ATG ACC CTG CTA CAT GCT C-3` 5`-ATC CCA CTC CAG CAT GAC-3` 53 1,5
Ex12 5`-CTC TTT GTG CAT ACG GAG-3` 5`-ATC CCT TCA AGT CAA TCC-3` 52 1,5
Ex13 5`-GGG ATT AAC TCT ATC TCC TT-3` 5`-TGT GTA TAA TTC CAT CCT GG-3` 52 1,5
Ex14 5`-GCG ACA AGA TTG AAA CTC-3` 5`-TCT TCT CTC CAC CAA CTG-3` 52 2,5
Ex15 5`-ATG CAC CAC ACT AAG GAA TG-3` 5`-CTC TAT GGC CCA AAC AGA G-3` 54 1,5
Ex16 5`-CCT GGT CCT TGA GAT TCT GA-3` 5`-CAG GTC TGC AAT GAC TTC-3` 52 2,5
Ex17 5`-CCT GGT CCT TGA GAT TCT GA-3` 5`-GAG GCT GGA TTG CTT GAT TC-3` 56 1,5