Convened under the auspicious of esteemed endorsers - ISTA

Saturday, October 9, 2010, 16:00-16:50Session A23: Knee MechanicsProgress in Developing a More Clincially Relevant Lubricant for in-VitroWear Testing of Total Knee Replacements*Jan-M. Brandt - Concordia Hip and Knee Institute - Winnipeg, CanadaKory Charron - London Health Sciences Centre - London, CanadaLin Zhao - University of Western Ontario - London, CanadaSteven MacDonald - London Health Sciences Centre - London, CanadaJohn Medley - University of Waterloo - Waterloo, Canada*Email: jbrandt@cjrg.caThere is no universal consensus on the protein constituent fractions, serum osmolality, additionof hyaluronate (HA) and microbial growth inhibitors for lubricants used in knee implant weartesting. The present study obtained clinical data on osteoarthritic synovial fluid (SF)composition, compared it with current lubricants and proposed a more clinically relevantlubricant.SF was drawn from 20 patients with osteoarthritis. The protein constituent fractions andosmolality were determined. Knee simulator (AMTI) wear tests for a total of 11.5 millioncycles were conducted on gas-plasma sterilized polyethylene (PE) inserts (AMK, GUR 1050;DePuy Orthopedics, Warsaw, IN) with 3 calf sera of different protein sub-constituents: alpha(ACS), newborn (NCS) and bovine (BCS). The effects of osmolality and hyaluronic acid (HA)on PE wear were also assessed. In addition, electrophoresis, protein degradation, microbialcontamination and protein shear were determined.The protein constituent fractions for NCS and BCS were largely different from SF; ACS wasclosest to SF. The wear rate was different between all three calf sera (p