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Cambridge International A Level Biology Revision Guide

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<strong>Cambridge</strong> <strong>International</strong> AS <strong>Level</strong> <strong>Biology</strong><br />

a What is a protoplast? [1]<br />

b i What is the pressure potential at 90%, 95% and 100% relative cell volume? [1]<br />

ii Calculate the change in pressure potential between 90% and 95% relative cell volume and between 95%<br />

and 100% relative cell volume. [2]<br />

iii Explain why the pressure potential curve is not linear. [2]<br />

iv State the water potential when the cell reaches maximum turgidity. [1]<br />

The graph above shows that as the cell loses water, pressure potential falls and the relative cell volume<br />

decreases (the cell shrinks).<br />

c i What is the minimum value of the pressure potential? [1]<br />

ii In a shrinking cell, what is the relative cell volume when the minimum value of the pressure potential<br />

is reached? [1]<br />

iii What is the term used to describe the state of the cell at this point? [1]<br />

iv What happens to the values of water potential and solute potential at this point? [1]<br />

v State the equation which links ψ p<br />

, ψ s<br />

and ψ. [1]<br />

vi Describe what is happening to the cell between the point identified in c ii and c iii above and 80%<br />

relative cell volume. [5]<br />

d As the cell changes volume, the change in solute potential is much less than the change in pressure potential.<br />

Suggest an explanation for this. [3]<br />

[Total: 20]<br />

12 The diagram shows the concentration in mmol dm –3 of two different ions inside a human red blood cell and<br />

in the plasma outside the cell.<br />

92<br />

ion<br />

blood<br />

plasma<br />

red blood cell<br />

Na +<br />

144<br />

K + 5<br />

15<br />

150<br />

a Explain why these concentrations could not have occurred as a result of diffusion. [1]<br />

b Explain how these concentrations could have been achieved. [2]<br />

c If respiration of red blood cells is inhibited, the concentrations of potassium ions and sodium ions inside<br />

the cells gradually change until they come into equilibrium with the plasma. Explain this observation. [4]<br />

[Total: 7]

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