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Cambridge International A Level Biology Revision Guide

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<strong>Cambridge</strong> <strong>International</strong> A <strong>Level</strong> <strong>Biology</strong><br />

450<br />

The Svalbard Global Seed Vault is a seed bank run by<br />

the government of Norway. The vault is at the end of a<br />

120 metre tunnel cut into the rock of a mountain on the<br />

island of Spitsbergen, within the Arctic Circle. The vault<br />

is thought to have ideal storage conditions, and it is only<br />

opened in winter when the environmental temperature<br />

falls close to the operating temperature of −18 °C. Its first<br />

seed samples went into storage in January 2008, and by<br />

2013 the vault held over 770 000 different seed samples of<br />

crop varieties from all over the world.<br />

National and international organisations collect and<br />

store seeds so that the genetic diversity in our crop plants<br />

is not lost. Seeds from each sample are stored in the vault<br />

at Svalbard. If seeds stored elsewhere are lost for any<br />

reason, such as an environmental disaster that destroys<br />

a seed bank, then there will always be duplicate samples<br />

available from Svalbard. Such problems could range from<br />

mismanagement or loss of funding to accidents or failure<br />

of equipment. The depositing seed bank owns its seeds<br />

and alone has access to them. Any research organisation<br />

wishing to use any of the seeds has to apply to the original<br />

seed bank for such seeds. The storage of the seeds is free of<br />

charge, as the costs of the upkeep of the vault are covered<br />

by the Norwegian government and by the Global Crop<br />

Diversity Trust. This trust also helps developing countries<br />

to select and package seeds that can then be sent to<br />

Svalbard to be stored.<br />

Another important seed bank is that of the <strong>International</strong><br />

Rice Institute in the Philippines, which holds all rice varieties.<br />

Many plants produce seeds, known as orthodox seeds,<br />

that remain viable for at least 15 years if they are carefully<br />

dehydrated until they contain only about 5% water, and<br />

then stored at around −15 to −20 °C. With this small water<br />

content, there is little danger that cells in the seed will be<br />

damaged by ice crystals during freezing and thawing.<br />

The only way to find out whether or not stored seeds<br />

are still viable is to try to germinate them. Seed banks<br />

carry out germination tests at five-year intervals. When<br />

fewer than 85% of the seeds germinate successfully, then<br />

plants are grown from these seeds so that fresh seed can be<br />

collected and stored.<br />

When such plants are grown from samples of stored<br />

seed, there is the possibility of altering the genetic diversity<br />

that was originally stored. Small samples of seeds from<br />

rare plants present a particular problem, as even smaller<br />

samples of the original are taken to test for viability or<br />

to grow into plants to increase the number of seeds in<br />

store. Such samples are unlikely to contain all the genetic<br />

diversity of the original sample. The only answer to this<br />

problem is to put as large and diverse a sample as possible<br />

into store in the first place.<br />

Most seeds are easy to store, but some plants have seeds<br />

that cannot be dried and frozen. These ʻrecalcitrant seedsʼ,<br />

as they are called, include seeds of economically important<br />

tropical species, such as rubber, coconut palm, coffee and<br />

cocoa (Figure 18.35). The only ways to keep the genetic<br />

diversity of these species are to collect seeds and grow<br />

successive generations of plants or to keep them as tissue<br />

culture. Cocoa is banked as trees. The <strong>International</strong> Cocoa<br />

Genebank in Trinidad has about 12 000 trees – examples<br />

of all the cocoa varieties found in Latin America and the<br />

Caribbean. Selected material from the Trinidad collection<br />

is distributed to other cocoa-producing countries after it<br />

has been through quarantine at the University of Reading<br />

in the UK.<br />

Coconut palms are particularly difficult to bank<br />

(Figure 18.36). The seed (the coconut) is very large, and<br />

the embryo is too large to freeze successfully. Collectors<br />

remove the embryos from the seeds, culture them in sterile<br />

tubes and eventually plant them.<br />

Figure 18.35 The future of cocoa, Theobroma cacao, is<br />

threatened by diseases, climate change, natural disasters,<br />

limited genetic diversity and the failure to manage plantations<br />

by replacing old trees. 30–40% of the world’s production is<br />

lost to pests and disease.

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