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Cambridge International A Level Biology Revision Guide

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<strong>Cambridge</strong> <strong>International</strong> A <strong>Level</strong> <strong>Biology</strong><br />

It is rare to try to catalogue everything. In an area of<br />

grassland or woodland, you might choose to concentrate<br />

on just one or two groups, such as flowering plants<br />

and insects. On a rocky shore, a study may involve the<br />

most obvious organisms such as seaweeds and molluscs<br />

(Worked example 3, page 432).<br />

There are now two questions to ask: how are the<br />

different species spread throughout the ecosystem and how<br />

many individuals of each species are there? The answers to<br />

these two questions describe what we call distribution<br />

and abundance.<br />

sample is random is to mark out an area with measuring<br />

tapes and use a random number generator, such as an app<br />

on a mobile phone. The random numbers give you the<br />

coordinates of the sampling points in relation to the two<br />

tapes you have used to mark out the area (Figure 18.8).<br />

428<br />

QUESTION<br />

18.3 Some students surveyed the species diversity of an<br />

area of woodland and some grassland nearby. They<br />

used a 20-minute timed search.<br />

a Explain why they used the same technique for the<br />

two areas.<br />

b They found 56 species in the woodland and 12 in<br />

the grassland. What other data could they have<br />

collected to compare the biodiversity of the two<br />

ecosystems?<br />

Sampling<br />

To find out which species are present in an ecosystem,<br />

and the size of the population of each of them, the ideal<br />

method would be to find, identify and count every single<br />

organism that lives there. We can sometimes do this if the<br />

area is very small or the species are very large. But it is only<br />

rarely possible. Instead, we take samples from the area we<br />

are interested in, and use these to make an estimate of the<br />

total numbers in the area.<br />

Sampling can be random or systematic. If an area<br />

looks reasonably uniform, or if there is no clear pattern<br />

to the way species are distributed, then it is best to use<br />

random sampling.<br />

Random sampling using quadrats<br />

A quadrat is a square frame that marks off an area of<br />

ground, or water, where you can identify the different<br />

species present and/or take a measurement of their<br />

abundance. You need to decide on a suitable size for the<br />

quadrat and how many samples you will take.<br />

Samples must be taken randomly to avoid any bias.<br />

For example, you might choose to take all of your samples<br />

from the place with fewest species simply because it is the<br />

easiest to do. This would not be representative of the whole<br />

area you are surveying. The usual way to ensure that a<br />

Figure 18.8 In random sampling, quadrats are positioned<br />

randomly in an area marked off by measuring tapes. This<br />

reduces the chances of bias in sampling the ecosystem.<br />

You can use your results in two different ways: to<br />

calculate species frequency and species density. Species<br />

frequency is a measure of the chance of a particular species<br />

being found within any one quadrat. You simply record<br />

whether the species was present in each quadrat that you<br />

analyse. For example, if you placed your quadrat 50 times,<br />

and found daisy plants in 22 of your samples, then the<br />

species frequency for daisies is:<br />

22 × 100 = 44%<br />

50<br />

Species density is a measure of how many individuals<br />

there are per unit area – for example, per square metre.<br />

The number of individuals that you have counted is<br />

divided by the total area of all your quadrats.<br />

It is not always possible to count individual plants and<br />

animals because of the way that they grow. For example,<br />

many animals and plants grow over surfaces forming a<br />

covering and it is almost impossible to count individuals.<br />

How do you decide how many grass plants there are in a<br />

quadrat that you have placed on a lawn? In this case, you<br />

can estimate the percentage cover of the species within<br />

your quadrat (Figure 18.9). To help with this, you can use<br />

a 100 cm × 100 cm quadrat with wires running across it at<br />

10 cm intervals in each direction, dividing the quadrat into

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