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Cambridge International A Level Biology Revision Guide

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Chapter 13: Photosynthesis<br />

QUESTION<br />

13.5 Some of the most productive crop plants in the<br />

world are C4 plants. However, rice grows in tropical<br />

regions and is a C3 plant. Research is taking place<br />

into the possibility of producing genetically modified<br />

rice that uses the C4 pathway in photosynthesis.<br />

Explain how this could increase yields from rice.<br />

Trapping light energy<br />

Chloroplasts contain several different pigments, and<br />

these different pigments absorb different wavelengths of<br />

light. The photosynthetic pigments of higher plants form<br />

two groups: the chlorophylls (primary pigments) and the<br />

carotenoids (accessory pigments) (Table 13.1).<br />

Group Pigment Colour<br />

chlorophylls<br />

carotenoids<br />

chlorophyll a<br />

chlorophyll b<br />

β carotene<br />

xanthophyll<br />

yellow-green<br />

blue-green<br />

orange<br />

yellow<br />

Table 13.1 The colours of the commonly occurring<br />

photosynthetic pigments.<br />

Chlorophylls absorb mainly in the red and blueviolet<br />

regions of the light spectrum. They reflect green<br />

light, which is why plants look green. The structure of<br />

chlorophyll a is shown in Figure 13.15. The carotenoids<br />

absorb mainly in the blue-violet region of the spectrum.<br />

CH CH 3<br />

CH 2<br />

CH 2 CH 3<br />

An absorption spectrum is a graph of the absorbance of<br />

different wavelengths of light by a pigment (Figure 13.16a).<br />

An action spectrum is a graph of the rate of<br />

photosynthesis at different wavelengths of light<br />

(Figure 13.16b). This shows the effectiveness of the<br />

different wavelengths, which is, of course, related to their<br />

absorption and to their energy content. The shorter the<br />

wavelength, the greater the energy it contains.<br />

a<br />

Absorbance<br />

Rate of photosynthesis<br />

chlorophyll a<br />

chlorophyll b<br />

carotenoids<br />

400 450 500 550 600 650 700<br />

Wavelength of light / nm<br />

b<br />

295<br />

head<br />

tail<br />

H 3 C<br />

H 3 C<br />

N N<br />

Mg<br />

N N<br />

(CH 2 ) 2<br />

C O C O<br />

O O<br />

CH 2 CH 3<br />

CH<br />

C CH 3<br />

(CH 2 ) 3<br />

CH CH 3<br />

(CH 2 ) 3<br />

CH CH 3<br />

(CH 2 ) 3<br />

CH CH 3<br />

CH 3<br />

O<br />

CH 3<br />

400 450 500 550 600 650 700<br />

Wavelength of light / nm<br />

Figure 13.16 a Absorption spectra of chlorophylls a and b,<br />

and carotenoid pigments. b Photosynthetic action spectrum.<br />

QUESTION<br />

13.6 Compare the absorption spectra shown in<br />

Figure 13.16a with the action spectrum shown<br />

in Figure 13.16b.<br />

a Identify and explain any similarities in the<br />

absorption and action spectra.<br />

b Identify and explain any differences between the<br />

absorption and action spectra.<br />

Figure 13.15 Structure of chlorophyll a. You do not need to<br />

learn this molecular structure.

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