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Cambridge International A Level Biology Revision Guide

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<strong>Cambridge</strong> <strong>International</strong> A <strong>Level</strong> <strong>Biology</strong><br />

504<br />

Substituting into this formula, we can calculate<br />

Spearman’s rank correlation coefficient for the distribution<br />

of species R and species S.<br />

(6 × 12)<br />

r s<br />

= 1 −<br />

(10 3 – 10)<br />

= 1 − 72<br />

1000 – 10<br />

= 1 − 72<br />

990<br />

= 1 − 0.072<br />

= 0.928<br />

= 0.93 (to 2 decimal places)<br />

This number is the correlation coefficient. The closer the<br />

value is to 1, the more likely it is that there is a genuine<br />

correlation between the two sets of data.<br />

Our value is very close to 1, so it certainly looks as<br />

though there is strong correlation. However, as with the<br />

t-test and the χ 2 test, we need to look up this value in a<br />

table, and compare it against a critical value. As in most<br />

statistical tests used in biology, we use a probability of<br />

0.05 as our baseline; if our value indicates a probability<br />

of 0.05 or less, then we can say that there is a significant<br />

correlation between our two samples. Another way of<br />

saying this is that the null hypothesis, that there is no<br />

correlation between the two samples, is not supported.<br />

Table P2.8 shows these critical values for samples with<br />

different numbers of readings.<br />

Notice that, the smaller the number of the readings we<br />

have taken, the larger our value of r s<br />

needs to be in order<br />

to say that there is a significant correlation. This makes<br />

logical sense – if we have taken only 5 readings, then we<br />

would really need them all to be ranked identically to be<br />

able to say they are correlated. If we have taken 16, then we<br />

can accept a smaller value of r s<br />

.<br />

Remember that showing there is a correlation between<br />

two variables does not indicate a causal relationship – in<br />

other words, we can’t say that the numbers of species R<br />

have an effect on the numbers of species S, or vice versa.<br />

There could well be other variables that are causing both<br />

of their numbers to vary.<br />

For our data, we have 10 quadrats, so n = 10. The<br />

critical value is therefore 0.65. Our value is much greater<br />

than this, so we can accept that there is a significant<br />

correlation between the numbers of species R and the<br />

numbers of species S.<br />

Table P2.9 summarises the circumstances in which<br />

you would use each of the statistical tests that help you to<br />

decide whether or not there is a relationship between two<br />

sets of data.<br />

Evaluating evidence<br />

It is important to be able to assess how much trust you can<br />

have in the data that you have collected in an experiment,<br />

and therefore how much confidence you can have in any<br />

conclusions you have drawn. Statistical tests are a big help<br />

in deciding this. But you also need to think about the<br />

experiment itself and any sources of error that might have<br />

affected your results.<br />

Sources of error were discussed in Chapter P1, on<br />

page 263. You will remember that sources of error stem<br />

from two main sources – limitations in the apparatus and<br />

measuring instruments, and difficulty in controlling key<br />

variables.<br />

In a written practical examination at A level, you<br />

will often be analysing data that you have not collected<br />

yourself, so you will have only the information provided<br />

in the question and your own experience of carrying out<br />

experiments to help you to decide how much confidence<br />

you can have in the reliability of the data. Important<br />

things to think about include the following questions.<br />

■■<br />

■■<br />

■■<br />

■■<br />

How well were the key variables controlled?<br />

Was the range and interval of the independent variable<br />

adequate?<br />

Do any of the results appear to be anomalous? If so,<br />

what could have caused these anomalous readings?<br />

Have the provided readings been replicated sufficiently?<br />

Another aspect of the experiment that needs to be<br />

considered is its validity. A valid experiment really does<br />

test the hypothesis or question that is being investigated.<br />

n 5 6 7 8 9 10 11 12 14 16<br />

Critical value of r s<br />

1.00 0.89 0.79 0.76 0.68 0.65 0.60 0.54 0.51 0.51<br />

Table P2.8 Critical values of r s<br />

at the 0.05 probability level.

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