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Cambridge International A Level Biology Revision Guide

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<strong>Cambridge</strong> <strong>International</strong> AS <strong>Level</strong> <strong>Biology</strong><br />

Answers to end-of-chapter questions<br />

(in this case) by osmosis;<br />

through partially permeable cell surface<br />

membrane of cell;<br />

as cell fills with water, cell / protoplast<br />

expands and pressure (potential) increases;<br />

until water potential of cell = zero / water<br />

potential of pure water;<br />

cell wall rigid / will not stretch (far), and<br />

prevents entry of more water;<br />

cell is turgid; [max. 5]<br />

[Total: 8]<br />

10a the greater the concentration difference, the<br />

greater the rate of transport;[1]<br />

b (net) diffusion and facilitated diffusion only<br />

occur if there is a concentration, difference /<br />

gradient, across the membrane<br />

or<br />

at equilibrium / if no concentration<br />

difference, there is no, net exchange /<br />

transport across membrane / rate of<br />

transport, is same in both directions; AW<br />

active transport can occur even if no<br />

concentration difference;<br />

because molecules / ions are being pumped;<br />

AW[3]<br />

c i active transport;[1]<br />

ii active transport depends on a supply of<br />

ATP;<br />

provided by respiration;[2]<br />

d graph for diffusion is linear / straight line<br />

(with no maximum rate);<br />

purely physical process / not dependent<br />

on transport proteins / channel or carrier<br />

proteins;<br />

graph for facilitated diffusion is a curve with a<br />

maximum rate; AW<br />

facilitated diffusion depends on presence<br />

of, transport proteins / channel or carrier<br />

proteins;<br />

as concentration increases, the receptor<br />

sites of these proteins become more and<br />

more saturated / the more saturated these<br />

become, the less the effect of increasing<br />

concentration;<br />

rate reaches a maximum when all, transport<br />

/ channel or carrier proteins, are working at<br />

full capacity / when all receptor sites are, full<br />

/ saturated;<br />

NB: This is similar to the effect of substrate<br />

concentration on rate of enzyme activity.<br />

[max. 5]<br />

[Total: 12]<br />

11a the living contents of a plant cell;[1]<br />

b i at 90% = 22 kPa (accept 21 or 23 kPa), at<br />

95% = 100 kPa, at 100% = 350 kPa;[1]<br />

ii change 90–95 % = 78 kPa (accept 77 or<br />

79 kPa);<br />

change 95–100% = 250 kPa;[2]<br />

iii as water enters the cell, the cell wall is<br />

stretched / protoplast pushes against cell<br />

wall;<br />

cell wall is (relatively) rigid;<br />

water cannot be compressed;<br />

therefore pressure builds up more and<br />

more rapidly (for given volume of water)<br />

/ small increase in amount of water has<br />

large effect on pressure; AW [max. 2]<br />

(This could be compared with pumping up<br />

a bicycle tyre – pressure increases much<br />

more rapidly for a given amount of air<br />

towards the end due to the elastic limit of<br />

the tyre being reached.)<br />

iv 350 kPa;[1]<br />

c i zero (kPa);[1]<br />

ii 86%;[1]<br />

iii incipient plasmolysis;[1]<br />

iv water potential = solute potential;[1]<br />

v ψ = ψ s + ψ p ;[1]<br />

vi the cell continues to lose water /<br />

protoplast continues to shrink;<br />

protoplast pulls away from cell wall =<br />

plasmolysis;<br />

shrinks until equilibrium is reached;<br />

when water potential of cell = water<br />

potential of outside solution;<br />

solute potential gets lower / more<br />

negative;<br />

because cell contents becoming more<br />

concentrated; [max. 5]<br />

d only a small amount of water is needed to<br />

bring about a large change in pressure;<br />

because the cell wall is (relatively) rigid;<br />

<strong>Cambridge</strong> <strong>International</strong> AS and A <strong>Level</strong> <strong>Biology</strong> © <strong>Cambridge</strong> University Press 2014

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