24/06/2005 - Controller General of Patents, Designs, and Trade Marks

(12) PATENT APPLICATION PUBLICATION
(19) INDIA (21)
(22) Date of filing of Application: 27/06/2003 (43)
(54) Title of the invention:
Application No: 657/MUMNP/2003 A
Publication Date: 24/06/2005
IN VITRO METHOD FOR TRANSFECTION OF EUKARYOTIC CELL FOR
PRODUCING DRUG FOR NON-VIRAL GENE THERAPY
(51) International Classification : C 12 N 15/87 (71)
(31) Priority Document No. : 101 00 996.8
(32) Priority Date : 10/01/2001
(33) Name of priority country : GERMANY
(86)
International Application
No.
: PCT/DE02/00060
Filing Date : 10/01/2002
(87) International Publication
No.
: WO 02/055721 A2
(61) Patent of addition to
Application No.
: NIL
Filed on : N.A.
(62) Divisional to Application
No.
: NIL
Filed on : N.A.
(57) Abstract:
The invention relates to a method for the transfection of cells using
at least one protein capable of forming nucleoprotein filaments. Said
protein is first modified by at least one functional constituent which
influences at least one step of the transfection, and the nucleic acid
to be transfected is then loaded with the modified protein. The
nucleic acid and the protein form a filamentary complex, and said
complex is then added to the cells to be transfected. The invention
also relates to a transfection agent of nucleoprotein filaments (NPF),
at least one protein forming a nucleoprotein filament being modified
by at least one functional constituent for the transfection. The
invention further relates to the use of the inventive transfection agent
for producing a medicament for the gene therapeutic treatment of
humans and animals. Finally, the invention relates to corresponding
pharmaceutical preparations, especially for use in gene therapy, and
the use of such transfection agents in the form of constituents in kits.
(72)
Name of the Applicant:
AMAXA GMBH
Address of the Applicant:
NATTERMANNALLEE 1,
50829 KOLN, GERMANY.
Name of the Inventors:
1. SCHMIDT HANNS-MARTIN
2. ALTROGGE LUDGER
3. LENZ DIETMAR
4. RIEMEN GUDULA
5. BROSTERHUS HELMUT
6. LORBACH ELKE
7. HELFRICH JULIANA
8. HEIN KATHARINA
9. GREMSE MARION
10. MALES TATJANA
11. CHRISTINE RAINER
12. SIEBENKOTTEN GREGOR
13. ORTMANN BODO
14. TURBANSKI TAMARA
15. KLAES ANDREA
Filed U/S 5(2) before the
Patents (Amendment)
Ordinance, 2004: NO
(FIG.: 20)
Total Pages: 77.
The Patent Office Journal 24.06.2005 17808