24/06/2005 - Controller General of Patents, Designs, and Trade Marks

(21) Application No. 27/DEL/2000 A (22) Date of filing of Application: 18/01/2000
(54) Title of the invention: “A PROCESS FOR THE BIOTRANSFORMATION OF
CYANOHYDROCARBONS TO CORRESPOONDING
AMIDES”
(51) International Classification 7 : C 12P013/02
(30) Priority Data:
(31) Document No.: NA
(32) Date: NA
(33) Name of convention country: NA
(66) Filed U/s 5(2): NIL
(61) Patent of addition to application No.: NA
(62) Filed on: NA
(63) Divisional to Application No.: NIL
(64) Filed on: NA
Total No of Pages: 20
(57) Abstract:
(71) Name of the applicant:
COUNCIL OF SCIENTIFIC AND
INDUSTRIAL RESEARCH
Address of the Applicant:
Rafi Marg, New Delhi-110001
(72) Name of the Inventor:
BINOD KUMAR GOGOI
RITU ARAMABAM
TANKESHESWAR NATH
AJIT KUMAR HAZARIKA
JAGDISH NARAYAN NIGAM
NARENDRA NATH DUTTA
The present invention relates to a process for the biotransformation of cyanohydrocarbons to
corresponding amides. It relates to a process for the biotransformation of cyanohydrocaaarbos to
corresponding amides using whole cell of a novel Rhodococcus sp. Strain RL4 having nitrile
hydratase enzyme. The present invention particularly relates to conversion of aliphatic and
aromatic cyanohyddrocarbons such as acrylonitrile, propionitrile, isobutyronitrile, benzyl cyanide,
3-cyanopyridine and the like to the corresponding amides, and more particularly relates to an
enzymatic process for the hydration of acrylonitrile to acrylamide scatalyzed by nitrile hyratase
present in the resting cells of Rhodococcus sp. RL4. The main usage of the invention is to
provide a process for the preparaton of Rhodococcus sp. RL4 cells having nitirile hydratase
enzyme which can be used under mild reaction conditions e.g. atmospheric pressure, low
temperature, near neutral pH to hydrate acrylonitrile to acrylamide without any by-product
formation.
18177