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Food additives data book - wordpres

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Enzymes 402<br />

NAME: Beta-glucanase<br />

CATEGORY: Enzymes<br />

FOOD USE: Hydrolyses cellulose in brewing worts<br />

SYNONYMS: Endo-1,3(4)-beta-D-glucanase/ EC 3.2.1.6/ CAS 9074-99-1 (b- from Aspergillus niger), 9012-54-8<br />

(from Bacillus subtilis)/ laminarinase/ EINECS 232-980-2, 232-734-4 resp.<br />

pH OPTIMUM: Aspergillus niger 5; Bacillus subtilis 7; Penicillium emersonii 4<br />

TEMPERATURE OPTIMUM °C: Aspergillus niger 60; Bacillus subtilis 50–60; Penicillium emersonii 70<br />

SIDE ACTIVITIES: Aspergillus niger amylase, glucoamylase, glucosidase; Bacillus subtilis amylase, glucosidase, protease;<br />

Penicillium emersonii amylase, dextranase, protease<br />

FUNCTION IN FOODS: Endo-hydrolysis of terminal 1,4-alpha-D-glucose residues from non-reducing end of polyglucoside<br />

chains. Enzyme for beer filtration<br />

TECHNOLOGY OF USE IN FOODS: Hydrolyses cellulose in brewing worts<br />

LEGISLATION: USA: UK and CANADA: AUSTRALIA/<br />

EUROPE: PACIFIC RIM:<br />

GRAS from Approved Ale, beer, light beer, malt liquor, porter, stout, Japan: approved<br />

Aspergillus niger corn for degerming distiller’s mash, mash<br />

destined for vinegar manufacture,<br />

unstandardised bakery products<br />

REFERENCES: Ash, M., and Ash, I. (1995) <strong>Food</strong> Additives. Gower Publishing Co., Brookfield, VT.<br />

Owusu-Ansah, Y. J. (1991) Enzymes. In: Smith, J. (Ed.), <strong>Food</strong> Additive User’s Hand<strong>book</strong>. Blackie<br />

A & P, London, pp. 120–150.<br />

ANY OTHER RELEVANT INFORMATION: Aspergillus niger broad range with low specificity; Bacillus subtilis narrow range with higher<br />

specificity; Penicillium emersonii tolerant of low pH in some variants

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