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Food additives data book - wordpres

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Enzymes 452<br />

NAME: Pullulanase<br />

CATEGORY: Enzymes<br />

FOOD USE: Sugar manufacture<br />

SYNONYMS: EC 3.2.1.4/ CAS 9075-68-7/ EINECS 232-983-9<br />

PROPERTIES AND APPEARANCE: White powder<br />

pH OPTIMUM: Bacillus spp. 4.5; Klebsiella aerogenes 5<br />

TEMPERATURE OPTIMUM °C: Bacillus spp. 60; Klebsiella aerogenes 50<br />

SIDE ACTIVITIES: Bacillus spp. protease, amylase; Klebsiella aerogenes protease, amylase<br />

FUNCTION IN FOODS: <strong>Food</strong> enzyme in production of maltose. Hydrolysis of 1,6-alpha-D-glucosidic link in pullulan,<br />

amylopectin, glycogen and limit dextrans<br />

TECHNOLOGY OF USE IN FOODS: <strong>Food</strong> enzyme in production of maltose. Hydrolysis of 1,6-alpha-D-glucosidic link in pullulan,<br />

amylopectin, glycogen and limit dextrans<br />

LEGISLATION: UK and EUROPE:<br />

Klebsiella aerogenes<br />

REFERENCES: Ash, M., and Ash, I. (1995) <strong>Food</strong> Additives. Gower Publishing Co., Brookfield, VT.<br />

Owusu-Ansah, Y. J. (1991) Enzymes. In: Smith, J. (Ed.), <strong>Food</strong> Additive User’s Hand<strong>book</strong>. Blackie<br />

A & P, London, pp. 120–150.

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