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Food additives data book - wordpres

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Enzymes 424<br />

NAME: Glucose isomerase<br />

CATEGORY: Enzymes<br />

FOOD USE: Conversion of glucose to fructose<br />

SYNONYMS: D-Xylose ketol isomerase/ EC 5.3.1.5<br />

pH OPTIMUM: Actinoplanes missouriensis 7.5; Bacillus coagulans 8; Streptomyces spp. 8; Streptomyces albus 6–7<br />

TEMPERATURE OPTIMUM °C: Actinoplanes missouriensis 60; Bacillus coagulans 60; Streptomyces spp. 63; Streptomyces albus 60–75<br />

SIDE ACTIVITIES: Actinoplanes missouriensis – usually none; Bacillus coagulans – usually none; Streptomyces spp. –<br />

usually none; Streptomyces albus – usually none.<br />

FUNCTION IN FOODS: A true xylose isomerase acting on glucose at high substrate concentration. Enzyme which converts<br />

glucose to fructose; used in production of high-fructose corn syrup<br />

TECHNOLOGY OF USE IN FOODS: Immobilised 0.0015–0.03% DS fixed bed 0.16 DS batch<br />

SYNERGISTS: In the immobilised form activated by magnesium and cobalt (the need for cobalt varies with<br />

preparation), magnesium is competed for by calcium and must therefore be in excess<br />

LEGISLATION: USA: UK and EUROPE:<br />

GRAS status – 184.1372 (immobilised preparations) – Soluble – Bacillus coagulans.<br />

Actinoplanes missouriensis; Bacillus coagulans; Immobilised – Bacillus<br />

Streptomyces olivaceous; Streptomyces murinus, coagulans, Streptomyces<br />

Streptomyces rubiginosus, Streptomyces olivochromogenes, olivaceous<br />

Arthrobacter globiformus<br />

AUSTRALIA/PACIFIC RIM:<br />

CANADA: Japan: approved<br />

Actinoplanes missouriensis; Bacillus coagulans;<br />

Streptomyces spp.; Olivochromogenes spp.; S. olivaceous.<br />

Glucose, glucose syrup partially or completely<br />

isomerised to fructose

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