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livre - Wiki do IF-SC

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Atividade Relativa (%)<br />

(a) (b)<br />

(c)<br />

Atividade Residual (%)<br />

120<br />

100<br />

80<br />

60<br />

40<br />

20<br />

0<br />

-20<br />

130<br />

120<br />

110<br />

100<br />

90<br />

80<br />

70<br />

60<br />

50<br />

40<br />

30<br />

20<br />

10<br />

0<br />

-10<br />

-20<br />

-30<br />

0 20 40 60 80 100<br />

(e)<br />

% etanol<br />

0 20 40 60 80 100<br />

% glutaraldeí<strong>do</strong><br />

Atividade Residual (%)<br />

120<br />

100<br />

80<br />

60<br />

40<br />

20<br />

0<br />

-20<br />

Atividade Residual (%)<br />

Atividade Residual (%)<br />

(d)<br />

0 20 40 60 80 100<br />

Figura 29: Efeito <strong>do</strong> solvente + tampão fosfato pH=4,0 (% v/v) sobre a atividade residual da enzima<br />

<strong>livre</strong> (símbolo aberto) e a enzima imobilizada (símbolo fecha<strong>do</strong>) à 25ºC.<br />

120<br />

100<br />

0 20 40 60 80 100<br />

80<br />

60<br />

40<br />

20<br />

0<br />

-20<br />

140<br />

120<br />

100<br />

80<br />

60<br />

40<br />

20<br />

-20<br />

-40<br />

% acetato de etila<br />

0<br />

% acetona<br />

0 20 40 60 80 100<br />

% dioxano<br />

93

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