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Principles of Plant Genetics and Breeding

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256 CHAPTER 14<br />

Part B<br />

Please answer the following questions:<br />

1 Give the full name for each <strong>of</strong> the following acronyms:<br />

RFLP: …….....………………………………………...<br />

AFLP: ………….....……………………………………<br />

SCAR: ……………....…………………………………<br />

2 Give two specific disadvantages <strong>of</strong> isozyme markers.<br />

3 Give two specific applications <strong>of</strong> molecular markers in plant breeding.<br />

4 What is marker-assisted selection?<br />

5 What is a transgenic organism?<br />

6 What is a transgene?<br />

7 Describe how microprojectile bombardment is used in biotechnology.<br />

8 Give two examples <strong>of</strong> scorable markers used in biotechnology.<br />

9 What is a promoter?<br />

10 Discuss the types <strong>of</strong> promoters used in genetic engineering.<br />

11 What is genomics?<br />

12 What is bioinformatics, <strong>and</strong> what is its role in biotechnology?<br />

Part C<br />

Please write a brief essay on each <strong>of</strong> the following topics:<br />

1 Give the rationale <strong>of</strong> markers.<br />

2 Discuss the use <strong>of</strong> markers in plant cultivar identification.<br />

3 Discuss the advantages <strong>of</strong> AFLP technology.<br />

4 Discuss how breeding GM cultivars differs from breeding conventional cultivars.<br />

5 Describe gene transfer by Agrobacterium mediation.<br />

6 Give the general characteristics <strong>of</strong> restriction endonucleases.<br />

7 Discuss plasmid vectors used in rDNA research.<br />

8 Describe the method <strong>of</strong> gene transfer by biolistics.

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