Zeitschrift für Rheumatologie – Supplement 1 - Deutsche ...

Die individuellen Verläufe der Höhe der CCP-AK korrelierten nicht
mit dem DAS28.
Diskussion: Die Höhe der CCP-AK korreliert schwach aber signifi -
kant mit der Krankheitsaktivität (DAS28), der Korrelationskoeffi zient
und das Signifi kanzniveau ist den bekannten Parametern (BKS, CRP,
RF) jedoch deutlich unterlegen. Auch im individuellen Verlauf ist der
CCP-AK nicht als Parameter zur Einschätzung des Krankheitsverlaufs
zu verwenden.
Eine regelmäßige Bestimmung der CCP-AK bei bekannter RF/CCP-
AK positiver rheumatoider Arthritis erscheint daher nicht sinnvoll.
Einfach zu bestimmende Parameter (MS, HAQ) bzw. kostengünstig
zu bestimmende Parameter (BKS) sind wesentlich besser zur Abschätzung
der Krankheitsaktivität geeignet.
POFR1-13
Association between ß2-adrenergic receptor polymorphisms in conjunction
with the HLA-DRB1 shared epitope and rheumatoid arthritis
Malysheva OA., Wagner U., Wahle M., Wagner U., Pierer M., Häntzschel H.,
Baerwald CG.
Uniklinik, Medizinische Klinik IV, Leipzig
Purpose: It can be suspected that inherited polymorphisms at codons
16, 27 and 164 of the beta2-adrenergic receptor (beta2R) gene as well
as HLA alleles may turn out to be important in the development and
maintenance of rheumatoid arthritis (RA). Th erefore, the frequency of
the main polymorphisms in the beta2R gene and the association with
HLA-DRB1 alleles and RA was examined.
Methods: An allele-specifi c polymerase chain reaction was used to determine
the common variants of the beta2R at position 16, 27, and 164
in patients with RA (n= 310) and ethnically matched healthy controls
(n= 305) from Germany. In a subgroup of RA patients and controls
HLA-DRB1 genotyping was performed by oligonucleotide hybridisation
of enzymatically amplifi ed DNA allowing low-resolution HLA-
DRB1 genotyping comprising specifi cities DRB1*01 to DRB1*17 and
the identifi cation of alleles pertinent to the assessment of the shared
epitope and the DERAA-positive DRB1 alleles.
Results: Th e age of the RA patients was 47.2 ± 9.8 years with disease
duration of 18.0 ± 3.4 years. 271 RA patients (87.4 %) were positive for
the rheumatoid factor. Th e distribution of the beta2R alleles in controls
and RA patients followed Mendelian inheritance. Arginine (Arg)
at codon 16 was present in 278 RA patients (89.7 %) compared to 202
controls (66.2%; OR 4.43, 95 % CI 2.81 to 7.02, p=0.00001). Homozygosity
for Arg16 was found in 107 RA patients (34.5 %) compared to 14
controls (4.6 %; OR 10.9, CI 5.9 to 20.5; p=0.00001). Homozygosity for
Glutamine (Gln) at codon 27 was present in 94 RA patients (30.3%)
compared to 67 controls (22%; OR 1.5, 95% CI 1.06-2.26, p=0.023).
Interestingly, the patients with the Arg16 allele have a younger age of
disease onset compared to patients without Arg16 (46,1±2,0 versus
53,1±2,7 respectively, p