Protocols for Micropropagation of Woody Trees and Fruits

IN VITRO SHOOT DEVELOPMENT OF TAXUS WALLICHIANA ZUCC.
Table 2. Concentrations of plant growth regulators α-naphthaleneacetic acid (NAA), indole-
3-acetic acid (IAA) and indole-3-butyric acid (IBA) and activated charcoal supplemented into
the half-strength MS basal medium used for shoot growth and elongation.
Medium NAA
(mg.l –1 IAA
) (mg.l –1 IBA
) (mg.l –1 Activated charcoal
)
(g.l –1 )
C1 1 – – –
C2 3 – – –
C3 5 – – –
C4 – 1 – –
C5 – 3 – –
C6 – 5 – –
C7 – – 1 –
C8 – – 3 –
C9 – – 5 –
C10 – – 2.5 2
C11 2.5 – – 2
Table 3. Concentrations of plant growth regulator (6-benzyladenine i.e. BA) and activated
charcoal supplemented into the half-strength MS basal medium used for adventitious bud
induction.
Medium Basal medium BA (mg.l –1 ) Activated charcoal
(g.l –1 )
AB1 ½ MS 1 –
AB2 ½ MS 3 –
AB3 ½ MS 5 –
AB4 ½ MS 1 2
Table 4. Concentrations of plant growth regulators α-naphthaleneacetic acid (NAA), indole-
3-acetic acid (IAA) and indole-3-butyric acid (IBA, vitamin B1 and activated charcoal supplemented
into the full- or half-strength MS basal medium used for root formation.
Basal NAA
medium (mg.l –1 IAA
) (mg.l –1 IBA
) (mg.l –1 Vitamin B1
) (mg.l –1 Activated
) charcoal (g.l –1 Medium
)
R1 MS – – 5 10 –
R2 MS 5 – – 10 –
R3 MS – 5 – 10 –
R4 ½ MS – – 5 10 –
R5 ½ MS 5 – – 10 –
R6 ½ MS – 5 – 10 –
R7 ¼ MS – – 5 10 –
R8 ¼ MS 5 – – 10 –
R9 ¼ MS – 5 – 10 –
R10 ½ MS – – 2.5 – 2
R11 ½ MS 2.5 – – – 2
R12 ½ MS – 2.5 – – 2
R13 ½ MS – – 2.5 – –
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