Protocols for Micropropagation of Woody Trees and Fruits

PROTOCOL FOR MICROPROPAGATION OF QUERCUS SPP.
2.3.5. Field Testing
In vitro derived Quercus spp. plants were after 2 years transferred to experimental
fields. From the beginning the plants were characterised by asynchronous growth,
what was manifested in differences in plant height. Later no obvious differences
were visible between in vitro and by cuttings propagated plants. The single plants
showed good morphology and physiological health, without morphological anomalies.
3. CONCLUSION
In vitro propagation techniques were proved to be perspective and suitable means of
effective propagation and reproduction of selected genotypes of Quercus spp., as well
as for elimination of problems connected with generative and classical vegetative
reproduction. It should be stated that for optimal cultivation and regeneration small
protocol modifications may be necessary in different Quercus species.
Acknowledgements. The work was supported by Slovak Grant Agency VEGA, project no.
2/6001/26.
4. REFERENCES
Lloyd, G. & McCown, B. (1980) Commercially feasible micropropagation of mountain laurel Kalmia
latifolia, by use of shoot tip culture. Proc. Int. Plant Propagators Soc. 30, 421–427.
Ostrolucká, M.G. & Bežo, M. (1994) Utilization of meristem cultures in propagation of oak (Quercus
spp.). Genet. Pol. 35, 161–169.
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