Protocols for Micropropagation of Woody Trees and Fruits

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E.C. NUNES ET AL.
2. Incubate the root cultures in the dark and the epicotyl cultures either in the
light or in the dark.
3. After 45 days the globular calli are collected to determine the fresh and dry
mass.
High callus fresh weight is obtained from root segments cultured on 1.15–2.3 µM 2,
4-D. The epicotyl segments cultured in the light produce superior values of callus
fresh weight at all concentrations of 2,4-D or at 2.7 µM NAA; in the dark the best
concentration for callus induction is either 4.6 µM 2,4-D or 2.7 µM NAA.
Table 3. Peak area percentage of the volatile constituents collected in the head space SPME
of callus of Cedrela fissilis cultured either in the light or in the dark during 8 weeks on MS
basal medium supplemented with 2.5 µM BAP and 5 µM NAA.
Compound R.I. a Callus
cultured in
the light
Callus
cultured in
the dark
Unknown 974 0.73 0.62
Limonene b
1031 0.67 1.33
1,8-Cineol b
1033 9.40 10.19
Terpinolene b
1088 0.72 0.99
Camphor b
1143 2.10 2.41
Borneol b
1165 1.10 1.12
α-Terpineol b 1189 3.95 4.50
Bornyl acetate b
1285 44.01 54.03
Tridecane d
1300 0.75 —
α-Terpenyl acetate b 1350 9.87 13.10
Tetradecane d
1400 1.72 0.79
Unknown 1410 1.18 1.37
Unknown 1482 0.65 —
Germacrene D c
1491 2.02 —
Unknown 1499 0.61 1.90
α-Alaskene c 1513 1.12 —
6,11-oxo-Acor-4-ene c
1531 4.01 3.92
Unknown 1615 2.65 0.8
Unknown 1717 10.32 1.19
Unknown 1893 1.57 —
Unknown 1900 0.84 —
a Retention Index, b monoterpenes, c sesquiterpenes, d alkanes