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Protocols for Micropropagation of Woody Trees and Fruits

Protocols for Micropropagation of Woody Trees and Fruits

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MICROPROPAGATION OF BLACK LOCUST 199<br />

research is, however, needed to obtain high quality micropropagated plants <strong>for</strong> af<strong>for</strong>estation<br />

<strong>and</strong> <strong>for</strong>est tree breeding purposes. Microporpagation technique is also important<br />

when utilizing somaclonal variation <strong>and</strong>/or gene engineering techniques. <strong>Micropropagation</strong><br />

may also be used to preserve gene resources <strong>of</strong> black locust <strong>and</strong> combined<br />

with tree breeding, it may improve industrial utilization <strong>of</strong> the black locust wood.<br />

4. REFERENCES<br />

Barghchi, M. & Chi, Y. H. (1998) In vitro regeneration <strong>and</strong> plant improvement in black locust (Robinia<br />

pseudoacacia L.). In tree biotechnology: towards the millennium. Nottingham University Press, pp.<br />

187–196.<br />

Davis, J.M. & Keathley, D.E. (1987) Differential responses to in vitro bud culture in mature Robinia<br />

pseudoacacia L. (black locust). Plant Cell Rep. 6, 431–434.<br />

Kamlesh, K., Sehgal, R.N. & Deepak, S. (1995) Effect <strong>of</strong> explant type on the micropropagation <strong>of</strong><br />

Robinia pseudoacacia. Indian J. For. 18, 47–52.<br />

Molnár, S. & Bariska, M. (2006) Wood species <strong>of</strong> Hungary. Budapest: Szaktudás Kiadó Ház, pp. 54-60.<br />

Murashige, T. & Skoog, F. (1962) A revised medium <strong>for</strong> rapid growth <strong>and</strong> bioassays with tobacco tissue<br />

cultures. Physiol. Plant 15, 473–497.<br />

Pan, Z.G. & You, Y.T. (1994) Growing exotic trees in China. Beijing: Beijing Science & Technology<br />

Press, pp. 462–472.<br />

Rédei, K. (1999) Improvement <strong>and</strong> Management in Hungary. Forestry Studies in China, Vol. 1, No. 2.<br />

Scowcr<strong>of</strong>t, W. R. (1985) Genetic flux in plants. Springer-Verlag, Wien New York, pp. 217–245.<br />

Shu, Q.Y. & Liu, G.S. (2003) An effective method <strong>for</strong> axillary bud culture <strong>and</strong> RAPD analysis <strong>of</strong> cloned<br />

plants in tetraploid black locust. Plant Cell Rep. 22, 175–180.

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