Protocols for Micropropagation of Woody Trees and Fruits

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DOUBLED-HAPLOID MICROPROPAGATION IN QUERCUS SUBER L. celltrics disposable filter. Small globular embryos, the proliferating tissue of the embryo base and the embryo axis without cotyledons provide a good material for the analysis of the ploidy level. The suspension of released nuclei was stained with 1500 µl staining solution (Partec Cystain UV precise P Kit) and left for 5 min. Figure 2. Procedures for induction and proliferation of gametic embryogenesis in cork oak (Quercus suber L.). A) Quercus suber L. tree. B) Branches with catkins. C) Cork oak anther cultures on induction medium supplemented with activated charcoal. D) Microspore embryogenesis from some cork oak anthers. E) Detail of small globular embryos emerging from inside the anther. F) Proliferation of cork oak haploid embryos. 167
168 B. PINTOS ET AL. Table 1. Formulation of culture medium used for doubled haploid micropropagation in Quercus suber L. based on modified Sommer et al. (1975) macronutrients and Murashige & Skoog (1962) micronutrients and augmented with culture stage-specific plant growth regulators. Components Chemical formula Stock (g/L) Macronutrients, 100× stock, use 10 ml per L medium Medium (mg/L) Potassium chloride KCl 30 300 Potassium nitrate KNO3 100 1000 Calcium chloride-2H2O CaCl2·2H2O 15 150 Magnesium sulfate-7H2O MgSO4·7H2O 25 250 Ammonium sulfate (NH4)2·SO4 20 200 Sodium phosphate NaH2PO4·2H2O 12.950 129.50 Micronutrients, 100 × stock, use 10 ml per L medium Potassium iodide KI 0.083 0.83 Boric acid H3BO3 0.62 6.2 Manganese sulfate-H2O MnSO4·H2O 1.69 16.9 Zinc sulfate-7H2O ZnSO4·7H2O 0.86 8.6 Sodium molybdate-2H2O Na2MoO4·2H2O 0.025 0.25 Cupric sulfate-5H2O CuSO4·5H2O 0.0025 0.025 Cobalt chloride-6H2O CoCl2·6H2O 0.0025 0.025 Iron –EDTA, 100 × stock, use 10 ml per L medium Iron sulfate-7H2O FeSO4·7H2O 2.78 27.8 Ethylenediamine tetraacetic acid disodium Na2EDTA 3.72 37.2 Vitamins, 100 × stock, use 10 ml per L medium Myo-Inositol 10 100 Nicotinic acid 0.05 0.5 Pyridoxine hydrochloride 0.05 0.5 Thiamine hydrochloride 0.01 0.1 Glycine 0.2 2 Ascorbic acid 0.2 2 Sucrose Other additives 30000 Agar 8000 Plant growth regulators, glutamine and activated charcoal, add according to culture stage Final concentrations (mg/L) Culture stage Glutamine Charcoal BAP IBA Induction medium – 10000 – – Proliferation medium 500 – – – Maturation medium – 10000 0.05 – Germination medium – – – 0.1
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PROTOCOLS FOR MICROPROPAGATION OF W
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A C.I.P. Catalogue record for this
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vi TABLE OF CONTENTS 14. Root induc
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viii TABLE OF CONTENTS 43. Micropro
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x PREFACE on precise stepwise proto
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CHAPTER 1 TOTIPOTENCY AND THE CELL
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TOTIPOTENCY AND THE CELL CYCLE 5 a
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2.2. Plant Bioregulators and the Ce
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TOTIPOTENCY AND THE CELL CYCLE 9 in
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TOTIPOTENCY AND THE CELL CYCLE 11 F
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Bartel, D. (2004) MicroRNAs: genomi
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CHAPTER 2 MICROPROPAGATION VIA ORGA
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MICROPROPAGATION OF SLASH PINE Tabl
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MICROPROPAGATION OF SLASH PINE Amon
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2.6. Field Testing MICROPROPAGATION
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CHAPTER 3 MICROPROPAGATION OF COAST
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MICROPROPAGATION OF SEQUOIA SEMPERV
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CHAPTER 4 MICROPROPAGATION OF PINUS
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MICROPROPAGATION OF PINUS PINEA L.
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42 2.1. Organ Culture K. ISHII ET A
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44 K. ISHII ET AL. scent light, 70
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46 Chemicals (mg/l) K. ISHII ET AL.
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48 Table 3. Effects of plant growth
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50 K. ISHII ET AL. Gupta, P.K. & Du
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52 C. HARGREAVES AND M. MENZIES (Me
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54 C. HARGREAVES AND M. MENZIES Con
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56 2.1.3. Organogenesis from Field-
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58 C. HARGREAVES AND M. MENZIES Fig
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60 C. HARGREAVES AND M. MENZIES Fig
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62 C. HARGREAVES AND M. MENZIES For
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64 C. HARGREAVES AND M. MENZIES and
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CHAPTER 7 GENETIC FIDELITY ANALYSES
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PLANT GENETIC FIDELITY 69 Plant mat
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e added to samples, as this fluoroc
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11. Determine the mean channel numb
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3. In addition to testing various b
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GeneScan internal size standards: 4
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3500 3000 2500 2000 1500 1000 500 0
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PLANT GENETIC FIDELITY 81 microprop
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PLANT GENETIC FIDELITY 83 Steinkell
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86 2.1. Explant Preparation M.G. OS
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88 WPM (Lloyd & McCown, 1980) Macro
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90 M.G. OSTROLUCKÁ ET AL. on the m
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CHAPTER 9 MICROPROPAGATION OF MEDIT
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2.1. Explant Preparation MICROPROPA
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MICROPROPAGATION OF MEDITERRANEAN C
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108 D.T. NHUT ET AL. Larue (1953) a
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110 D.T. NHUT ET AL. HgCl2 added wi
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112 2.4. Establishment of Shoot Cul
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114 D.T. NHUT ET AL. Table 5. Effec
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Page 128 and 129: CHAPTER 12 MICROPROPAGATION OF LARI
Page 130 and 131: MICROPROPAGATION OF LARIX SPECIES V
Page 140 and 141: CHAPTER 13 PROPAGATION OF SELECTED
Page 142 and 143: PROPAGATION OF SELECTED PINUS GENOT
Page 150 and 151: CHAPTER 14 ROOT INDUCTION OF PINUS
Page 152 and 153: ROOT INDUCTION OF PINUS SYLVESTRIS
Page 154 and 155: ROOT INDUCTION OF PINUS SYLVESTRIS
Page 156 and 157: CHAPTER 15 MICROPROPAGATION OF BETU
Page 158 and 159: MICROPROPAGATION OF BETULA PENDULA
Page 166 and 167: CHAPTER 16 PROTOCOL FOR DOUBLED-HAP
Page 168 and 169: DOUBLED-HAPLOID MICROPROPAGATION IN
Page 182 and 183: CHAPTER 17 IN VITRO PROPAGATION OF
Page 184 and 185: IN VITRO PROPAGATION OF FRAXINUS SP
Page 188 and 189: Axillary shoots (number) IN VITRO P
Page 196 and 197: CHAPTER 18 MICROPROPAGATION OF BLAC
Page 198 and 199: MICROPROPAGATION OF BLACK LOCUST 19
Page 200 and 201: 2.5. Rooting and Acclimatization MI
Page 202 and 203: MICROPROPAGATION OF BLACK LOCUST 19
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Page 208 and 209: 206 V. RAJESWARI AND K. PALIWAL 2.3
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Page 212 and 213: 210 2.8. Hardening V. RAJESWARI AND
Page 214 and 215: CHAPTER 20 MICROPROPAGATION OF SALI
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Page 218 and 219: MICROPROPAGATION OF SALIX CAPREA L.
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MICROPROPAGATION OF SALIX CAPREA L.
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CHAPTER 21 MICROPROPAGATION OF CEDR
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2.1. Establishment of Shoot Culture
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MICROPROPAGATION OF CEDRELA FISSILI
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238 programmes is somatic embryogen
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240 Figure 2. A) Induction of organ
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242 2.4.1. Rooting of Apical and Ba
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244 of donor individuals and/or by
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246 J. MALÀ ET AL. Karnosky, D.F.,
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CHAPTER 23 MICROGRAFTING IN GRAPEVI
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MICROGRAFTING IN GRAPEVINE 251 and
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MICROGRAFTING IN GRAPEVINE 253 Tabl
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2.4. Culture Conditions MICROGRAFTI
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MICROGRAFTING IN GRAPEVINE 257 Feuc
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CHAPTER 24 MICROGRAFTING GRAPEVINE
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MICROGRAFTING GRAPEVINE FOR VIRUS I
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CHAPTER 25 APRICOT MICROPROPAGATION
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APRICOT MICROPROPAGATION Figure 1.
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APRICOT MICROPROPAGATION Figure 2.
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APRICOT MICROPROPAGATION 2.2.2. Hyp
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2.4. Acclimatization APRICOT MICROP
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APRICOT MICROPROPAGATION occurs fre
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CHAPTER 26 IN VITRO CONSERVATION AN
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IN VITRO CONSERVATION AND MICROPROP
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CHAPTER 27 MICROGRAFTING OF PISTACH
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MICROGRAFTING OF PISTACHIO Constitu
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MICROGRAFTING OF PISTACHIO 2.2.2. C
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MICROGRAFTING OF PISTACHIO 6. Incub
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MICROGRAFTING OF PISTACHIO 9. The r
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CHAPTER 28 PROTOCOL FOR MICROPROPAG
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MICROPROPAGATION OF CASTANEA SATIVA
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CHAPTER 29 MICROPROPAGATION OF CASH
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MICROPROPAGATION OF CASHEW 2.2.1. E
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MICROPROPAGATION OF CASHEW axillary
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MICROPROPAGATION OF CASHEW Table 1.
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MICROPROPAGATION OF CASHEW shade an
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CHAPTER 30 IN VITRO MUTAGENESIS AND
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IN VITRO MUTAGENESIS AND MUTANT MUL
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336 R.I. IYER compounds (Iyer et al
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A 338 2.2. Culture Medium R.I. IYER
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340 R.I. IYER Figure 2. Formation o
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342 R.I. IYER Figure 3. Formation o
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344 R.I. IYER Rao, Y.S., Mathew, K.
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346 B.K. BISWAS AND S.C. GUPTA marg
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348 B.K. BISWAS AND S.C. GUPTA (iv)
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350 B.K. BISWAS AND S.C. GUPTA 2.4.
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352 B.K. BISWAS AND S.C. GUPTA Figu
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354 B.K. BISWAS AND S.C. GUPTA tree
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356 B.K. BISWAS AND S.C. GUPTA Figu
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358 B.K. BISWAS AND S.C. GUPTA Drew
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CHAPTER 33 MICROPROPAGATION PROTOCO
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MICROPROPAGATION FOR MICROSPORE EMB
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374 L. TIAN AND S.I. SIBBALD younge
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376 L. TIAN AND S.I. SIBBALD Table
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378 L. TIAN AND S.I. SIBBALD 2.4. R
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CHAPTER 35 MICROPROPAGATION OF JUGL
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MICROPROPAGATION OF JUGLANS REGIA L
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CHAPTER 36 TISSUE CULTURE PROPAGATI
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PROPAGATION OF MONGOLIAN CHERRY AND
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410 M. PASQUAL AND E.A. FERREIRA 2.
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414 M. PASQUAL AND E.A. FERREIRA ch
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418 D.T. NHUT ET AL. its economical
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420 D.T. NHUT ET AL. Table 1. Shoot
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422 D.T. NHUT ET AL. Figure 3. Orga
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424 D.T. NHUT ET AL. mg l -1 NAA +
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426 D.T. NHUT ET AL. Nakasone, H.Y.
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428 C. LIU ET AL. C. cujete L. is c
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430 C. LIU ET AL. Table 1. Composit
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432 C. LIU ET AL. 6. Excise the sho
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434 Fresh weight per plantlet (mg)
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436 C. LIU ET AL. 4. REFERENCES Aga
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438 M. MISHRA ET AL. micropropagati
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440 M. MISHRA ET AL. each plate ino
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Section C
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446 M.G. OSTROLUCKÁ ET AL. from me
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448 M.G. OSTROLUCKÁ ET AL. regener
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450 M.G. OSTROLUCKÁ ET AL. Figure
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452 M.G. OSTROLUCKÁ ET AL. 2.6.3.
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454 counts counts M.G. OSTROLUCKÁ
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CHAPTER 42 PROTOCOL FOR MICROPROPAG
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AN medium (Anderson, 1980) MICROPRO
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MICROPROPAGATION OF VACCINIUM VITIS
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2.6. Flow Cytometry Analysis MICROP
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CHAPTER 43 MICROPROPAGATION OF BAMB
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BAMBOO MICROPROGATION BY AXILLARY S
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CHAPTER 44 IN VITRO CULTURE OF TREE
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IN VITRO CULTURE OF TREE PEONY 479
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500 M. MHATRE from various natural
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502 2.2. Disinfection of Plant Mate
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504 M. MHATRE soil in polythene bag
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506 M. MHATRE Figure 2. Pineapple,
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508 M. MHATRE Escalona, M., Lorenzo
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510 J.M. AL-KHAYRI Tunisia, Morocco
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512 J.M. AL-KHAYRI 2.1.2. Separatio
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514 2.2. Culture Medium J.M. AL-KHA
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516 J.M. AL-KHAYRI 5. Isolate callu
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518 J.M. AL-KHAYRI 3. Water the tra
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520 J.M. AL-KHAYRI expressed from c
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522 J.M. AL-KHAYRI alcohol and twic
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524 J.M. AL-KHAYRI potential. Simil
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526 J.M. AL-KHAYRI Barreveld, W.H.
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528 D.T. NHUT ET AL. arsenide chip
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530 D.T. NHUT ET AL. Figure 2. Plan
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532 D.T. NHUT ET AL. Figure 4. Plan
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534 D.T. NHUT ET AL. plantlets. The
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536 D.T. NHUT ET AL. Figure 11. Fre
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538 D.T. NHUT ET AL. Figure 13. Sub
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540 D.T. NHUT ET AL. The response o
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CHAPTER 48 IN VITRO MUTAGENESIS IN
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IN VITRO MUTAGENESIS IN BANANA 545
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3.3. Observations to be Recorded IN
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