Protocols for Micropropagation of Woody Trees and Fruits

MICROPROPAGATION OF SELECTED VACCINIUM SPP. 455
originated from meristems and/or adventitious buds shown that tested clones are
characterized by significant genetic stability, as flow cytometry analyses detected any
changes in ploidy level among tested clones (Gajdošová et al., 2006).
According flow cytometry analyses, as well as a low DNA polymorphism level
and distance index values determined by DNA microsatellite polymorphism it can
be stated that the presented regeneration protocol is suitable for production of true to
type plants.
3. CONCLUSION
In conclusion we can state, that an efficient cloning protocol was developed for
Vaccinium corymbosum L. cultivars, which enables large-scale propagation of high
quality, true to type plants for need of practice. Our results, as well as results of
other authors, prevailingly confirmed that in vitro regeneration ability is highly
genotype depending, therefore small protocol modifications may be necessary in
different cultivars.
Acknowledgement. The work was supported by Slovak Grant Agency VEGA, project no.
2/5128/25, COST 863 Action and APVT–20–026002.
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