Protocols for Micropropagation of Woody Trees and Fruits

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IN VITRO CONSERVATION AND MICROPROPAGATION OF BREADFRUIT Figure 2. Flow cytometry analysis of nuclei of leaf tissue from three breadfruit accessions (Puou, Maafala, and Puupuu). Internal standard control was not used in these samples. The 2C and 4C peaks reflect G0/G1 and G2/M stages. Puou and Maafala are diploids whereas Puupuu is a triploid. 287
288 S.J. MURCH ET AL. 4. REFERENCES Adebowale, K.O., Olu-Oqolabi, B.I., Olawumi, E.K. & Lawal, O.S. (2005) Functional properties of native, physically and chemically modified breadfruit (Artocarpus artilis) starch. Indust. Crops Prod. 21, 343–351. Amin, M.N. & Jaiswal, V.S. (1993) In vitro response of apical bud explants from mature trees of jackfruit (Artocarpus heterophyllus). Plant Cell Tiss. Org. Cult. 33, 59–65. Arumuganathan, K. & Earle, E.D. (1991a) Nuclear DNA content of some important plant species by flow cytometry. Plant Mol. Biol. Rep. 9, 208–218. Arumunganathan, K. & Earle, E.D. (1991b) Estimation of nuclear DNA contents of plant species by flow cytometry. Plant Mol. Biol. Rep. 9, 229–241. Bino, R.J., Devries, J.N., Kraak, H.L. & Van Pijlen, J.G. (1992) Flow cytometric determination of nuclear replication stages in tomato seeds during priming and germination. Ann. Bot. 69, 231–236. Fowler, C., Moore, G. & Hawtin, G. (2003) The International Treaty on Plant Genetic Resources for Food and Agriculture: A Primer for the Future Harvest Centers of the CGIAR. International Plant Genetic Resources Institute, Rome, Italy. Morton, J. (1987) Breadfruit. In Morton, J.F. (Ed.) Fruits of Warm Climates. Miami, FL. pp. 50–58. Murch, S.J., Ragone, D., Shi, W.L., Alan, A.R. & Saxena, P.K. (2007) In vitro conservation and sustained production of breadfruit (Artocarpus altilis, Moraceae): Modern technologies to improve distribution of a traditional tropical crop. Naturwissenchaften (in press). Ragone, D. (1997) Breadfruit. Artocarpus altilis (Parkinson) Fosberg. Promoting the conservation and use of underutilized and neglected crops. 10. International Plant Genetic Resources Institute, Rome, Italy. Ragone, D. (2001) Chromosome numbers and pollen stainability of three species of Pacific island breadfruit (Artocarpus, Moraceae). Amer. J. Bot. 88, 693–696. Ragone, D. (2003) Breadfruit. In Caballero, B., Trugo, L. & Finglas, P. (Eds) Encyclopedia of Food Sciences and Nutrition. Academic Press, San Diego pp. 655–661. Ramdath, D.D., Isaacs, R.L.C., Teelucksingh, S. & Wolever, T.M.S. (2004) Glycaemic index of selected staples commonly eaten in the Caribbean and the effects of boiling v. crushing. Brit. J. Nutr. 91, 971– 977. Zerega, N.J.C., Ragone, D. & Motley, T.J. (2004) Complex origins of breadfruit (Artocarpus altilis, Moraceae): Implications for human migrations in Oceania. Amer. J. Bot. 91, 760–766. Zerega, N.J.C., Ragone, D. & Motley, T.J. (2005) Systematics and species limits of breadfruit (Artocarpus, Moraceae). Systematic Botany 30, 603–615. Zerega, N.J.C., Ragone, D. & Motley, T.J. (2006) Breadfruit origins, diversity, and human-facilitated distribution. In Darwin’s Harvest: New Approaches to the Origins, Evolution, and Conservation of Crops. Columbia University Press. New York pp. 213–238.
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PROTOCOLS FOR MICROPROPAGATION OF W
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A C.I.P. Catalogue record for this
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vi TABLE OF CONTENTS 14. Root induc
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viii TABLE OF CONTENTS 43. Micropro
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x PREFACE on precise stepwise proto
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CHAPTER 1 TOTIPOTENCY AND THE CELL
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TOTIPOTENCY AND THE CELL CYCLE 5 a
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2.2. Plant Bioregulators and the Ce
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TOTIPOTENCY AND THE CELL CYCLE 9 in
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TOTIPOTENCY AND THE CELL CYCLE 11 F
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Bartel, D. (2004) MicroRNAs: genomi
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CHAPTER 2 MICROPROPAGATION VIA ORGA
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MICROPROPAGATION OF SLASH PINE Tabl
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MICROPROPAGATION OF SLASH PINE Amon
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2.6. Field Testing MICROPROPAGATION
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CHAPTER 3 MICROPROPAGATION OF COAST
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MICROPROPAGATION OF SEQUOIA SEMPERV
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CHAPTER 4 MICROPROPAGATION OF PINUS
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MICROPROPAGATION OF PINUS PINEA L.
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42 2.1. Organ Culture K. ISHII ET A
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44 K. ISHII ET AL. scent light, 70
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46 Chemicals (mg/l) K. ISHII ET AL.
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48 Table 3. Effects of plant growth
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50 K. ISHII ET AL. Gupta, P.K. & Du
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52 C. HARGREAVES AND M. MENZIES (Me
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54 C. HARGREAVES AND M. MENZIES Con
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56 2.1.3. Organogenesis from Field-
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58 C. HARGREAVES AND M. MENZIES Fig
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60 C. HARGREAVES AND M. MENZIES Fig
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62 C. HARGREAVES AND M. MENZIES For
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64 C. HARGREAVES AND M. MENZIES and
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CHAPTER 7 GENETIC FIDELITY ANALYSES
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PLANT GENETIC FIDELITY 69 Plant mat
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e added to samples, as this fluoroc
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11. Determine the mean channel numb
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3. In addition to testing various b
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GeneScan internal size standards: 4
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3500 3000 2500 2000 1500 1000 500 0
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PLANT GENETIC FIDELITY 81 microprop
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PLANT GENETIC FIDELITY 83 Steinkell
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86 2.1. Explant Preparation M.G. OS
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88 WPM (Lloyd & McCown, 1980) Macro
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90 M.G. OSTROLUCKÁ ET AL. on the m
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CHAPTER 9 MICROPROPAGATION OF MEDIT
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2.1. Explant Preparation MICROPROPA
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MICROPROPAGATION OF MEDITERRANEAN C
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108 D.T. NHUT ET AL. Larue (1953) a
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110 D.T. NHUT ET AL. HgCl2 added wi
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112 2.4. Establishment of Shoot Cul
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114 D.T. NHUT ET AL. Table 5. Effec
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116 D.T. NHUT ET AL. culture to be
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118 D. EWALD ability of the plant m
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120 D. EWALD Figure 1. Yew micropro
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122 D. EWALD Root development. Afte
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CHAPTER 12 MICROPROPAGATION OF LARI
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MICROPROPAGATION OF LARIX SPECIES V
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CHAPTER 13 PROPAGATION OF SELECTED
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PROPAGATION OF SELECTED PINUS GENOT
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CHAPTER 14 ROOT INDUCTION OF PINUS
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ROOT INDUCTION OF PINUS SYLVESTRIS
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ROOT INDUCTION OF PINUS SYLVESTRIS
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CHAPTER 15 MICROPROPAGATION OF BETU
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MICROPROPAGATION OF BETULA PENDULA
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CHAPTER 16 PROTOCOL FOR DOUBLED-HAP
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DOUBLED-HAPLOID MICROPROPAGATION IN
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CHAPTER 17 IN VITRO PROPAGATION OF
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IN VITRO PROPAGATION OF FRAXINUS SP
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Axillary shoots (number) IN VITRO P
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CHAPTER 18 MICROPROPAGATION OF BLAC
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MICROPROPAGATION OF BLACK LOCUST 19
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2.5. Rooting and Acclimatization MI
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MICROPROPAGATION OF BLACK LOCUST 19
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202 V. RAJESWARI AND K. PALIWAL tha
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204 V. RAJESWARI AND K. PALIWAL Mak
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206 V. RAJESWARI AND K. PALIWAL 2.3
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208 V. RAJESWARI AND K. PALIWAL Fig
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210 2.8. Hardening V. RAJESWARI AND
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CHAPTER 20 MICROPROPAGATION OF SALI
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MICROPROPAGATION OF SALIX CAPREA L.
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CHAPTER 21 MICROPROPAGATION OF CEDR
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2.1. Establishment of Shoot Culture
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MICROPROPAGATION OF CEDRELA FISSILI
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Page 240 and 241: 240 Figure 2. A) Induction of organ
Page 242 and 243: 242 2.4.1. Rooting of Apical and Ba
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Page 248 and 249: CHAPTER 23 MICROGRAFTING IN GRAPEVI
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Page 252 and 253: MICROGRAFTING IN GRAPEVINE 253 Tabl
Page 254 and 255: 2.4. Culture Conditions MICROGRAFTI
Page 256 and 257: MICROGRAFTING IN GRAPEVINE 257 Feuc
Page 258 and 259: CHAPTER 24 MICROGRAFTING GRAPEVINE
Page 260 and 261: MICROGRAFTING GRAPEVINE FOR VIRUS I
Page 266 and 267: CHAPTER 25 APRICOT MICROPROPAGATION
Page 268 and 269: APRICOT MICROPROPAGATION Figure 1.
Page 270 and 271: APRICOT MICROPROPAGATION Figure 2.
Page 272 and 273: APRICOT MICROPROPAGATION 2.2.2. Hyp
Page 274 and 275: 2.4. Acclimatization APRICOT MICROP
Page 276 and 277: APRICOT MICROPROPAGATION occurs fre
Page 278 and 279: CHAPTER 26 IN VITRO CONSERVATION AN
Page 280 and 281: IN VITRO CONSERVATION AND MICROPROP
Page 288 and 289: CHAPTER 27 MICROGRAFTING OF PISTACH
Page 290 and 291: MICROGRAFTING OF PISTACHIO Constitu
Page 292 and 293: MICROGRAFTING OF PISTACHIO 2.2.2. C
Page 294 and 295: MICROGRAFTING OF PISTACHIO 6. Incub
Page 296 and 297: MICROGRAFTING OF PISTACHIO 9. The r
Page 298 and 299: CHAPTER 28 PROTOCOL FOR MICROPROPAG
Page 300 and 301: MICROPROPAGATION OF CASTANEA SATIVA
Page 312 and 313: CHAPTER 29 MICROPROPAGATION OF CASH
Page 314 and 315: MICROPROPAGATION OF CASHEW 2.2.1. E
Page 316 and 317: MICROPROPAGATION OF CASHEW axillary
Page 318 and 319: MICROPROPAGATION OF CASHEW Table 1.
Page 320 and 321: MICROPROPAGATION OF CASHEW shade an
Page 322 and 323: CHAPTER 30 IN VITRO MUTAGENESIS AND
Page 324 and 325: IN VITRO MUTAGENESIS AND MUTANT MUL
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A 338 2.2. Culture Medium R.I. IYER
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340 R.I. IYER Figure 2. Formation o
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342 R.I. IYER Figure 3. Formation o
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344 R.I. IYER Rao, Y.S., Mathew, K.
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346 B.K. BISWAS AND S.C. GUPTA marg
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348 B.K. BISWAS AND S.C. GUPTA (iv)
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350 B.K. BISWAS AND S.C. GUPTA 2.4.
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352 B.K. BISWAS AND S.C. GUPTA Figu
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354 B.K. BISWAS AND S.C. GUPTA tree
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356 B.K. BISWAS AND S.C. GUPTA Figu
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358 B.K. BISWAS AND S.C. GUPTA Drew
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CHAPTER 33 MICROPROPAGATION PROTOCO
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MICROPROPAGATION FOR MICROSPORE EMB
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374 L. TIAN AND S.I. SIBBALD younge
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376 L. TIAN AND S.I. SIBBALD Table
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378 L. TIAN AND S.I. SIBBALD 2.4. R
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CHAPTER 35 MICROPROPAGATION OF JUGL
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MICROPROPAGATION OF JUGLANS REGIA L
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CHAPTER 36 TISSUE CULTURE PROPAGATI
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PROPAGATION OF MONGOLIAN CHERRY AND
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410 M. PASQUAL AND E.A. FERREIRA 2.
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414 M. PASQUAL AND E.A. FERREIRA ch
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418 D.T. NHUT ET AL. its economical
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420 D.T. NHUT ET AL. Table 1. Shoot
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422 D.T. NHUT ET AL. Figure 3. Orga
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424 D.T. NHUT ET AL. mg l -1 NAA +
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426 D.T. NHUT ET AL. Nakasone, H.Y.
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428 C. LIU ET AL. C. cujete L. is c
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430 C. LIU ET AL. Table 1. Composit
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432 C. LIU ET AL. 6. Excise the sho
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434 Fresh weight per plantlet (mg)
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436 C. LIU ET AL. 4. REFERENCES Aga
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438 M. MISHRA ET AL. micropropagati
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440 M. MISHRA ET AL. each plate ino
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Section C
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446 M.G. OSTROLUCKÁ ET AL. from me
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448 M.G. OSTROLUCKÁ ET AL. regener
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450 M.G. OSTROLUCKÁ ET AL. Figure
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452 M.G. OSTROLUCKÁ ET AL. 2.6.3.
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454 counts counts M.G. OSTROLUCKÁ
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CHAPTER 42 PROTOCOL FOR MICROPROPAG
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AN medium (Anderson, 1980) MICROPRO
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MICROPROPAGATION OF VACCINIUM VITIS
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2.6. Flow Cytometry Analysis MICROP
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CHAPTER 43 MICROPROPAGATION OF BAMB
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BAMBOO MICROPROGATION BY AXILLARY S
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CHAPTER 44 IN VITRO CULTURE OF TREE
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IN VITRO CULTURE OF TREE PEONY 479
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500 M. MHATRE from various natural
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502 2.2. Disinfection of Plant Mate
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504 M. MHATRE soil in polythene bag
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506 M. MHATRE Figure 2. Pineapple,
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508 M. MHATRE Escalona, M., Lorenzo
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510 J.M. AL-KHAYRI Tunisia, Morocco
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512 J.M. AL-KHAYRI 2.1.2. Separatio
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514 2.2. Culture Medium J.M. AL-KHA
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516 J.M. AL-KHAYRI 5. Isolate callu
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518 J.M. AL-KHAYRI 3. Water the tra
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520 J.M. AL-KHAYRI expressed from c
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522 J.M. AL-KHAYRI alcohol and twic
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524 J.M. AL-KHAYRI potential. Simil
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526 J.M. AL-KHAYRI Barreveld, W.H.
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528 D.T. NHUT ET AL. arsenide chip
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530 D.T. NHUT ET AL. Figure 2. Plan
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532 D.T. NHUT ET AL. Figure 4. Plan
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534 D.T. NHUT ET AL. plantlets. The
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536 D.T. NHUT ET AL. Figure 11. Fre
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538 D.T. NHUT ET AL. Figure 13. Sub
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540 D.T. NHUT ET AL. The response o
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CHAPTER 48 IN VITRO MUTAGENESIS IN
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IN VITRO MUTAGENESIS IN BANANA 545
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3.3. Observations to be Recorded IN
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