Protocols for Micropropagation of Woody Trees and Fruits

THIN CELL LAYER TECHNOLOGY AND YELLOW PASSION FRUIT 423
2.6. Ex Vitro Acclimatization
Plantlets (3.5–4.0 cm tall) with healthy roots were removed from culture flasks.
They were washed gently with running tap water to remove agar sticking to the roots
and transplanted into plastic pots filled with soil and sand (ratio of 1:1, v/v) (Figure
5H). For the first 2 weeks, plantlets were kept under natural light, 75% relative
humidity, and irrigated with tap water three times a day, and kept acclimatizing in
the same condition but irrigated once a day for another 4 weeks before transferring
into the field.
Figure 4. Effects of BA and GA 3 on shoot elongation of yellow passion fruit after 2 and 3
months. a 1, a 2) ½MS + 5 mg l –1 BA + 4 mg l –1 GA 3; b 1, b 2) ½MS + 5 mg l –1 BA + 5 mg l –1 GA 3;
c1, c 2) ½MS + 5 mg l –1 BA + 3 mg l –1 GA 3; d 1, d 2) ½MS + 5 mg l –1 BA + 4 mg l –1 GA 3.
3. CONCLUSION
In this protocol, tTCL is efficient in enhancing shoot regeneration rate and number
of shoots per explant of yellow passion fruit. The optimal PGR for shoot formation
from tTCL explants was 1.0 mg l –1 BA. Shoot elongation should be carried out on
the culture medium containing 5 mg l –1 BA, and 6 mg l –1 GA3. NAA and IAA, each
added at the concentration of 1 mg l –1 in the MS medium were highly suitable for
rooting. The callus formation from shoot could be induced by culturing shoots in the
culture media containing high concentration of NAA.