Protocols for Micropropagation of Woody Trees and Fruits

MICROPROPAGATION OF PINUS PINEA L.
Figure 1. A) Isolated cotyledons of Pinus pinea after 6 days on caulogenic induction medium.
B) Cotyledons after 6 days on caulogenic induction medium and 35 days on caulogenic expression
medium. C) Buds isolated from cotyledons after 70 days on caulogenic expression
medium. D) Shoot multiplication after 16 weeks on caulogenic expression medium.
Hardening. After 3 weeks, plantlets were transferred to greenhouse in normal
humidity conditions (Figure 2B). Plantlets were fertilized every three months using
Basacote 3M (Compo). The temperature in the greenhouse ranged between 18–28°C.
After two months in nursery, the survival rate of microplants can reach 98%. No
plagiotrophic growth was observed and plants showed a well-developed root system
capable of sustaining further shoot outgrowth.
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