Protocols for Micropropagation of Woody Trees and Fruits

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THIMMAPPAIAH ET AL.
microshoots are given a slow dip in 250 ppm of either NAA or IAA for 48 h in dark
and potting the treated shoots in mixture of soil rite and sand (2:1 v/v). The duration
of rooting is longer (3–4 months) in the latter method. Spontaneous rooting up to
30% is also observed on shoots cultured on medium for elongation.
Figure 1. Micropropagation of cashew. A) In vitro germination of seedling. B) Axillary shoot
bud proliferation in nodal explant. C) Axillary shoot bud proliferation in cotyledonary node.
D) Shoot bud elongation. E) In vitro rooted shoots being hardened. F) Potted micropropagated
plants in the greenhouse. G) Micropropagated plant in the field. H) Micropropagated plant in
bearing. I) Fruits harvested from micropropagated plants.