Protocols for Micropropagation of Woody Trees and Fruits

THIN CELL LAYER TECHNOLOGY AND YELLOW PASSION FRUIT 421
Table 2. Shoot elongation media.
Treatments Medium BA (mg l –1 ) GA3 (mg l –1 )
EM1
EM2
EM3
EM4
1/2MS
1/2MS
1/2MS
1/2MS
Table 3. Rooting media.
Treatment Medium NAA (mg l –1 ) IAA (mg l –1 ) Sucrose (g l –1 )
MR1
MS
0
0
30
MR2
MS
0.5
0
30
MR3
MS
1.0
0
30
MR4
MS
2.0
0
30
MR5
½MS
0
0
30
MR6
½MS 1.0
0
30
MR7
MS
0
1.0
20
The shoot elongation was best observed on the medium containing 5 mg l –1 BA and
6 mg l –1 GA3 (EM4). Higher concentration of GA3 in the culture medium resulted in
longer shoots (Table 4). Shoots elongated rapidly and grew 3–4 cm long after 8
weeks of culture (Figure 4C,D). The elongation of explants cultured on EM1 and
EM2 media was only observed after 3 months of culture on ½ MS medium containning
3 mg l –1 GA3 and 5 mg l –1 BA.
Treatments
Table 4. Effects of BA and GA3 on shoot elongation.
Average of shoot length (cm)
EM1
2.0
EM2
0.6
EM3
4.1
EM4
4.7
2.5. Root Formation
Shoots, 3–4 cm long were placed on the rooting media (Table 3) for 6 weeks to
induce roots. The results indicated that all hormone-free media (MR1 and MR5)
could not induce rooting (Figure 5A, E). Various rooting responses of tested shoots
were recorded in both full strength MS and half-strength MS medium. All explants
were rooted in MS medium containing 1.0 mg l –1 NAA (MR3) (Figure 5C), reduced
of macro-nutrients in this medium results in lower root formation rate. In MR2,
MR4, and MR6 media, all explants exhibited light yellowish green callus with roots
(Figure 5B,D,F). No shoot formation was observed on MR4 and MR6 media. Shoots
exhibited vigorous roots without callus formation phase when cultured on MS
medium contained 1.0 mg l –1 IAA (Figure 5G).
5
5
5
5
3
4
5
6