Protocols for Micropropagation of Woody Trees and Fruits

APRICOT MICROPROPAGATION
Figure 2. (A) Leaf rosette after 2 weeks in culture ready for the first subculture. (B) Elongated
shoot.
Culture medium. The shoot proliferation culture medium, supplemented with 3%
sucrose and solidified with 0.6% agar, is also used for the in vitro introduction and
establishment of axillary shoots (Hispanlab, S.A.). The PGRs used in the
introduction medium are 1.76 µM BA and 0.2 µM indole-3-butyric acid (IBA). The
medium is dispensed in 25 × 150 mm culture tubes covered with permeable membrane
caps. Sterilization of media and culture conditions are described above. Axillary
buds sprout in this medium (Figure 3C, D) and, after removing contaminated nodes,
elongated shoots are transferred to jars containing proliferation medium (Figure 4).
Figure 3. In vitro introduction of apricot by axillary shoots. (A) New branches of apricot
growing during spring (B) Node explant introduced in the culture medium. (C and D)
Sprouting of axillary buds.
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