Protocols for Micropropagation of Woody Trees and Fruits

MICROGRAFTING OF PISTACHIO
6. Incubate the cultures in a growth room at 40 µmol m –2 s –1 photosynthetic
photon flux intensity (fluorescent lamps, 75W) and a photoperiod of 16 h at
25 ± 2°C for 10–14 days. Use the seedlings (Figure 1B) as rootstocks for
grafting.
2.2.4. Development of Grafting Method
Various grafting procedures were tried within the culture vessels without removing
the rootstocks from the medium (Figure 2):
1. Slit micrografting: The rootstock was decapitated to remove all leaves and
a vertical slit was made on the stump, and the scion base, cut in v-shape,
was fitted in the slit.
2. Wedge micrografting on the stump: The rootstock was decapitated to remove
all leaves. A wedge was cut in the stump, and the scion base cut in a v
shape, was gently fit into the wedge.
3. Wedge micrografting in the leaf axil: The rootstock was decapitated leaving
a single leaf. A wedge was cut off in the leaf axil after removing the leaf
blade and the scion base gently sloped to fit into the wedge.
similar to the seed germination medium. In the subsequent experiments, slit micrografting
was elected for grafting. In this method, the rootstock was decapitated to
remove all leaves and a vertical slit was made on the stump, and the scion base, cut
in v-shape, was inserted in the slit (Figure 1C).
Different scion sizes were tested: 10 mm long.
There were differences in the frequencies of successful micrografts between the
treatments applied (Onay et al., 2004b). Success increased with the size of the scions
up to 6 mm but then decreased with size. All shoot tips