Protocols for Micropropagation of Woody Trees and Fruits

PROPAGATION OF MONGOLIAN CHERRY AND NANKING CHERRY
shoot proliferation. Approximately 3–5 good quality, 16–20 mm long shoots, with
well developed leaves (15–25 mm), are produced after 4 weeks in culture (Figure 3).
The 8–15 µM level of BA in the medium is generally considered high, although it
has been reported to be optimal for a number of related species including Prunus ×
Hally Jolivette (Lineberger, 1983), Prunus cerasifera × Prunus munsoniana rootstocks
(Dalzotto & Docampo, 1997) and P. virginiana (Pruski et al., 2000). Thiadizuron (TDZ)
Figure 2. A) and B) Initiation of Mongolian cherry cultures, C) Proliferating cultures of
Nanking cherry, D) In vitro rotted culture of Nanking cherry (black fruit selection, BNC).
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