Protocols for Micropropagation of Woody Trees and Fruits

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DOUBLED-HAPLOID MICROPROPAGATION IN QUERCUS SUBER L. Tree allele (bp) Figure 9. Microsatellite (SSR) composition of the parent tree showing heterozygous genotype and of embryo samples, showing only one allele per locus. Allele sizes in bp. Number of antherinduced embryos bearing each allele (SsrQpZAG15, SsrQpZAG46, SsrQpZAG110) detected in cork oak tree. * 1:1 segregation at the 0.05 significance level (chi-squared test). 4. REFERENCES Bueno, M.A., Astorga, R. & Manzanera, J.A. (1992a) Plant regeneration through somatic embryogenesis in Quercus suber. Physiol. Plant. 85, 30–34. Bueno, M.A. & Manzanera, J.A. (1992b) Primeros ensayos de inducción de embriones somáticos de Quercus suber L. Scientia gerundensis. 18, 29–37. Bueno, M.A., Gómez, A., Boscaiu, M., Manzanera, J.A. & Vicente, O. (1997) Stress-induced formation of haploid plants through anther culture in cork oak (Quercus suber). Physiol. Plant. 99, 335–341. Bueno, M.A. & Manzanera, J.A. (2003) Oak anther culture. In Maluszynski, M., Kasha, K.J., Foster, B.P. & Szarejko, I. (Eds). Doubled Haploid Production in Crops Plants. A Manual. Kluwer Academic Publishers, Dordrecht. ISBN 1-4020-1544-5, pp. 297–301. 120 124 Tree allele (bp) 190 192 Tree allele (bp) 222 238 ANTHER A B C D --- 6 6 --- --- 6 1 5 ANTHER A B C D --- 6 4 2 --- 6 6 --- ANTHER A B C D 6 --- --- 6 6 --- 6 --- 177 Total No. embryos 7 17 Total No. embryos 10* 14* Total No. embryos 18 6
178 B. PINTOS ET AL. Bueno, M.A., Gomez, A., Sepúlveda, F., Segui, J.M., Testillano, P.S., Manzanera, J.A. & Risueño, M.C. (2003) Microspore-derived from Quercus suber anthers mimic zygotic embryos and maintain haploidy in long-term anther culture. J. Plant Physiol. 160, 953– 960. Bueno, M.A., Pintos, B., Prado, M.J., Gómez, A. & Manzanera, J.A. (2004) Androgenesis: A tool for woody plant breeding. In Pandalai, S.G. (Ed). Recent Research Developments in Genetics & Breeding. Vol. 1 Part II Research Signpost. ISBN: 81-7736-218-6, pp. 365–383. Doyle, J.J. & Doyle, J.L. (1990) Isolation of plant DNA from fresh tissue. Focus 12, 13–15. Gómez, A., Pintos, B., Aguiriano, E., Manzanera, J.A. & Bueno, M.A. (2001a) SSR markers for Quercus suber tree identification and embryo analysis. Journal of Heredity. 92, 292–295. Gómez, A., Pintos, B., Aguiriano, E., Manzanera, J.A. & Bueno, M.A. (2001b) Microspore embryogenesis in Quercus suber L.: Genetic evaluation. In Bohanec, B. (Ed). Biotechnological Approaches for Utilization of Gametic Cells. COST 824. Office for Official Publications of the European Communities. Luxembourg, pp. 171–176. Murashige, T. & Skoog, F. (1962) A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol. Plant. 15, 473–497. Pintos, B. (2005) Embriogénesis gamética y obtención de doble-haploides en alcornoque (Quercus suber L.). Tesis Doctoral. Departamento de Biología Vegetal I. Facultad de Ciencias Biológicas. Universidad Complutense de Madrid. Pintos, B, Manzanera, J.A. & Bueno, M.A. (2005) Cytological analysis of early microspore divisions and embryo formation in vitro anther culture of cork oak. Acta Physiologiae Plantarum. 27, 703–708. Sommer, H.E., Brown, C.L. & Kormanik, P.P. (1975) Differentiation of plantlets in longleaf pine (Pinus palustris Mill.) tissue cultured in vitro. Bot. Gaz. 136, 196–200. Ziegenhagen, B., Guillemaut, P. & Scholz, F. (1993) A procedure for mini-preparations of genomic DNA from needles of silver fir (Abies alba Mill.). Plant Mol. Biol. Rep. 11, 117–121.
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PROTOCOLS FOR MICROPROPAGATION OF W
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A C.I.P. Catalogue record for this
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vi TABLE OF CONTENTS 14. Root induc
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viii TABLE OF CONTENTS 43. Micropro
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x PREFACE on precise stepwise proto
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CHAPTER 1 TOTIPOTENCY AND THE CELL
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TOTIPOTENCY AND THE CELL CYCLE 5 a
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2.2. Plant Bioregulators and the Ce
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TOTIPOTENCY AND THE CELL CYCLE 9 in
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TOTIPOTENCY AND THE CELL CYCLE 11 F
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Bartel, D. (2004) MicroRNAs: genomi
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CHAPTER 2 MICROPROPAGATION VIA ORGA
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MICROPROPAGATION OF SLASH PINE Tabl
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MICROPROPAGATION OF SLASH PINE Amon
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2.6. Field Testing MICROPROPAGATION
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CHAPTER 3 MICROPROPAGATION OF COAST
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MICROPROPAGATION OF SEQUOIA SEMPERV
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CHAPTER 4 MICROPROPAGATION OF PINUS
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MICROPROPAGATION OF PINUS PINEA L.
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42 2.1. Organ Culture K. ISHII ET A
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44 K. ISHII ET AL. scent light, 70
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46 Chemicals (mg/l) K. ISHII ET AL.
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48 Table 3. Effects of plant growth
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50 K. ISHII ET AL. Gupta, P.K. & Du
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52 C. HARGREAVES AND M. MENZIES (Me
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54 C. HARGREAVES AND M. MENZIES Con
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56 2.1.3. Organogenesis from Field-
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58 C. HARGREAVES AND M. MENZIES Fig
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60 C. HARGREAVES AND M. MENZIES Fig
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62 C. HARGREAVES AND M. MENZIES For
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64 C. HARGREAVES AND M. MENZIES and
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CHAPTER 7 GENETIC FIDELITY ANALYSES
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PLANT GENETIC FIDELITY 69 Plant mat
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e added to samples, as this fluoroc
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11. Determine the mean channel numb
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3. In addition to testing various b
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GeneScan internal size standards: 4
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3500 3000 2500 2000 1500 1000 500 0
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PLANT GENETIC FIDELITY 81 microprop
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PLANT GENETIC FIDELITY 83 Steinkell
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86 2.1. Explant Preparation M.G. OS
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88 WPM (Lloyd & McCown, 1980) Macro
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90 M.G. OSTROLUCKÁ ET AL. on the m
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CHAPTER 9 MICROPROPAGATION OF MEDIT
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2.1. Explant Preparation MICROPROPA
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MICROPROPAGATION OF MEDITERRANEAN C
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108 D.T. NHUT ET AL. Larue (1953) a
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110 D.T. NHUT ET AL. HgCl2 added wi
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112 2.4. Establishment of Shoot Cul
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114 D.T. NHUT ET AL. Table 5. Effec
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116 D.T. NHUT ET AL. culture to be
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118 D. EWALD ability of the plant m
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120 D. EWALD Figure 1. Yew micropro
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122 D. EWALD Root development. Afte
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CHAPTER 12 MICROPROPAGATION OF LARI
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Page 140 and 141: CHAPTER 13 PROPAGATION OF SELECTED
Page 142 and 143: PROPAGATION OF SELECTED PINUS GENOT
Page 150 and 151: CHAPTER 14 ROOT INDUCTION OF PINUS
Page 152 and 153: ROOT INDUCTION OF PINUS SYLVESTRIS
Page 154 and 155: ROOT INDUCTION OF PINUS SYLVESTRIS
Page 156 and 157: CHAPTER 15 MICROPROPAGATION OF BETU
Page 158 and 159: MICROPROPAGATION OF BETULA PENDULA
Page 166 and 167: CHAPTER 16 PROTOCOL FOR DOUBLED-HAP
Page 168 and 169: DOUBLED-HAPLOID MICROPROPAGATION IN
Page 182 and 183: CHAPTER 17 IN VITRO PROPAGATION OF
Page 184 and 185: IN VITRO PROPAGATION OF FRAXINUS SP
Page 188 and 189: Axillary shoots (number) IN VITRO P
Page 196 and 197: CHAPTER 18 MICROPROPAGATION OF BLAC
Page 198 and 199: MICROPROPAGATION OF BLACK LOCUST 19
Page 200 and 201: 2.5. Rooting and Acclimatization MI
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Page 204 and 205: 202 V. RAJESWARI AND K. PALIWAL tha
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Page 216 and 217: MICROPROPAGATION OF SALIX CAPREA L.
Page 222 and 223: CHAPTER 21 MICROPROPAGATION OF CEDR
Page 224 and 225: 2.1. Establishment of Shoot Culture
Page 226 and 227: MICROPROPAGATION OF CEDRELA FISSILI
Page 228 and 229: MICROPROPAGATION OF CEDRELA FISSILI
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MICROPROPAGATION OF CEDRELA FISSILI
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238 programmes is somatic embryogen
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240 Figure 2. A) Induction of organ
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242 2.4.1. Rooting of Apical and Ba
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244 of donor individuals and/or by
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246 J. MALÀ ET AL. Karnosky, D.F.,
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CHAPTER 23 MICROGRAFTING IN GRAPEVI
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MICROGRAFTING IN GRAPEVINE 251 and
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MICROGRAFTING IN GRAPEVINE 253 Tabl
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2.4. Culture Conditions MICROGRAFTI
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MICROGRAFTING IN GRAPEVINE 257 Feuc
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CHAPTER 24 MICROGRAFTING GRAPEVINE
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MICROGRAFTING GRAPEVINE FOR VIRUS I
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CHAPTER 25 APRICOT MICROPROPAGATION
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APRICOT MICROPROPAGATION Figure 1.
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APRICOT MICROPROPAGATION Figure 2.
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APRICOT MICROPROPAGATION 2.2.2. Hyp
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2.4. Acclimatization APRICOT MICROP
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APRICOT MICROPROPAGATION occurs fre
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CHAPTER 26 IN VITRO CONSERVATION AN
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IN VITRO CONSERVATION AND MICROPROP
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CHAPTER 27 MICROGRAFTING OF PISTACH
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MICROGRAFTING OF PISTACHIO Constitu
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MICROGRAFTING OF PISTACHIO 2.2.2. C
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MICROGRAFTING OF PISTACHIO 6. Incub
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MICROGRAFTING OF PISTACHIO 9. The r
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CHAPTER 28 PROTOCOL FOR MICROPROPAG
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MICROPROPAGATION OF CASTANEA SATIVA
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CHAPTER 29 MICROPROPAGATION OF CASH
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MICROPROPAGATION OF CASHEW 2.2.1. E
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MICROPROPAGATION OF CASHEW axillary
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MICROPROPAGATION OF CASHEW Table 1.
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MICROPROPAGATION OF CASHEW shade an
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CHAPTER 30 IN VITRO MUTAGENESIS AND
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IN VITRO MUTAGENESIS AND MUTANT MUL
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336 R.I. IYER compounds (Iyer et al
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A 338 2.2. Culture Medium R.I. IYER
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340 R.I. IYER Figure 2. Formation o
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342 R.I. IYER Figure 3. Formation o
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344 R.I. IYER Rao, Y.S., Mathew, K.
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346 B.K. BISWAS AND S.C. GUPTA marg
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348 B.K. BISWAS AND S.C. GUPTA (iv)
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350 B.K. BISWAS AND S.C. GUPTA 2.4.
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352 B.K. BISWAS AND S.C. GUPTA Figu
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354 B.K. BISWAS AND S.C. GUPTA tree
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356 B.K. BISWAS AND S.C. GUPTA Figu
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358 B.K. BISWAS AND S.C. GUPTA Drew
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CHAPTER 33 MICROPROPAGATION PROTOCO
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MICROPROPAGATION FOR MICROSPORE EMB
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374 L. TIAN AND S.I. SIBBALD younge
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376 L. TIAN AND S.I. SIBBALD Table
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378 L. TIAN AND S.I. SIBBALD 2.4. R
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CHAPTER 35 MICROPROPAGATION OF JUGL
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MICROPROPAGATION OF JUGLANS REGIA L
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CHAPTER 36 TISSUE CULTURE PROPAGATI
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PROPAGATION OF MONGOLIAN CHERRY AND
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410 M. PASQUAL AND E.A. FERREIRA 2.
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414 M. PASQUAL AND E.A. FERREIRA ch
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418 D.T. NHUT ET AL. its economical
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420 D.T. NHUT ET AL. Table 1. Shoot
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422 D.T. NHUT ET AL. Figure 3. Orga
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424 D.T. NHUT ET AL. mg l -1 NAA +
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426 D.T. NHUT ET AL. Nakasone, H.Y.
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428 C. LIU ET AL. C. cujete L. is c
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430 C. LIU ET AL. Table 1. Composit
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432 C. LIU ET AL. 6. Excise the sho
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434 Fresh weight per plantlet (mg)
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436 C. LIU ET AL. 4. REFERENCES Aga
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438 M. MISHRA ET AL. micropropagati
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440 M. MISHRA ET AL. each plate ino
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Section C
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446 M.G. OSTROLUCKÁ ET AL. from me
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448 M.G. OSTROLUCKÁ ET AL. regener
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450 M.G. OSTROLUCKÁ ET AL. Figure
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452 M.G. OSTROLUCKÁ ET AL. 2.6.3.
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454 counts counts M.G. OSTROLUCKÁ
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CHAPTER 42 PROTOCOL FOR MICROPROPAG
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AN medium (Anderson, 1980) MICROPRO
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MICROPROPAGATION OF VACCINIUM VITIS
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2.6. Flow Cytometry Analysis MICROP
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CHAPTER 43 MICROPROPAGATION OF BAMB
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BAMBOO MICROPROGATION BY AXILLARY S
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CHAPTER 44 IN VITRO CULTURE OF TREE
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IN VITRO CULTURE OF TREE PEONY 479
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500 M. MHATRE from various natural
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502 2.2. Disinfection of Plant Mate
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504 M. MHATRE soil in polythene bag
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506 M. MHATRE Figure 2. Pineapple,
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508 M. MHATRE Escalona, M., Lorenzo
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510 J.M. AL-KHAYRI Tunisia, Morocco
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512 J.M. AL-KHAYRI 2.1.2. Separatio
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514 2.2. Culture Medium J.M. AL-KHA
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516 J.M. AL-KHAYRI 5. Isolate callu
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518 J.M. AL-KHAYRI 3. Water the tra
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520 J.M. AL-KHAYRI expressed from c
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522 J.M. AL-KHAYRI alcohol and twic
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524 J.M. AL-KHAYRI potential. Simil
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526 J.M. AL-KHAYRI Barreveld, W.H.
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528 D.T. NHUT ET AL. arsenide chip
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530 D.T. NHUT ET AL. Figure 2. Plan
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532 D.T. NHUT ET AL. Figure 4. Plan
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534 D.T. NHUT ET AL. plantlets. The
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536 D.T. NHUT ET AL. Figure 11. Fre
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538 D.T. NHUT ET AL. Figure 13. Sub
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540 D.T. NHUT ET AL. The response o
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CHAPTER 48 IN VITRO MUTAGENESIS IN
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IN VITRO MUTAGENESIS IN BANANA 545
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3.3. Observations to be Recorded IN
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