Protocols for Micropropagation of Woody Trees and Fruits

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A. GAJDOŠOVÁ ET AL.
Influence of cytokinin zeatin on shoot regeneration ability was evaluated after 5
weeks. Data evaluation was performed using Statgraphic analysis of variance and
multiple range analysis. The optimal composition of shoot regeneration medium and
zeatin concentration for the tested cultivar is presented in Table 1.
Positive influence of zeatin on multiple shoot induction was confirmed in
V. vitis-idaea. From tested zeatin concentrations the most effective was 0.75 mg l
zeatin (Figure 1A). On an average 6.8 numbers of shoots in cultivar ‘Red Pearl’
were regenerated on the medium supplemented with 0.75 mg l –1 zeatin (Ostrolucká
et al., 2002).
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D
B
Figure 1. In vitro propagation of V. vitis-idaea. A) Shoot regeneration from dormant apical
and axillary buds on AN medium with 0.75 mg l –1 zeatin. B) Adventitious shoot regeneration
on leaf-derived callus after 8 weeks of cultivation on AN medium with 0.5 mg l –1 zeatin. C)
Adventitious shoot regeneration on AN medium with 0.5 mg l –1 zeatin after 12 weeks of
cultivation. D) Intensive shoot proliferation on AN medium with 0.5 mg l –1 zeatin. E) In vitro
rooting of V. vitis-idaea, cv.‛Red Pearl’ microshoot.
2.3.2. Shoot Regeneration via Adventitious Organogenesis in Vaccinium vitis-idaea L.
Adventitious organogenesis is very efficient to scale up clonal production of selected
genotypes of several Vaccinium species (Gajdošová et al., 2006). Vaccinium vitisidaea
L. cv. ‘Red Pearl’ was tested for adventitious shoot induction. As a primary
explants leaves of in vitro plants with cut margins, were placed horizontally with
adaxial surface on the culture medium. AN medium supplemented with 2.19 mg l –1
zeatin was used for adventitious bud induction from leaf tissue (Table 1). After 5
weeks of cultivation explants were transferred on AN medium containing 0.5 mg l –1
zeatin for shoot regeneration and multiplication. Percentages of explants regenerating
shoots and number of regenerated shoots per explant after 3 subcultures, with
subculture interval 5 weeks, were recorded. Shoot regeneration via callus phase was
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C
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