Protocols for Micropropagation of Woody Trees and Fruits

506
M. MHATRE
Figure 2. Pineapple, Serial Micropropagation: scooping of dormant axillary bud from
crowns A), culture of bud on BSM solid medium B), sprouting of dormant axillary bud C),
proliferation in solid SMM D), proliferation in SMM liquid medium E), rooting of isolated
shoot in RM F), transfer to papercups with Soilrite G), field transfer of in vitro produced
plants H), fruiting I), harvested fruits J).
2.8. Pretreatment and Encapsulation Matrix
Prior to encapsulation the shoots should be soaked in liquid White’s (1954) basal
medium supplemented with sucrose (1%), NAA (10.8 µM ) and IBA (39.4 µM) and
agitated at 100 rpm (on a gyratory shaker) for 48 h. The shoots can be blot dried on
a filter paper, mixed in a gel of 3% sodium alginate (Sigma) prepared in MS basal
medium or SMM and encapsulated using CaCl2·2H2O (1.36g / 150 ml) solution.