Protocols for Micropropagation of Woody Trees and Fruits

270
O. PÉREZ-TORNERO AND L. BURGOS
Table 1. Macronutrients, micronutrients, vitamins and organics compounds of the culture
media used in apricot micropropagation (concentrations in mg/l).
Introduction and
establishment of
meristems
Shoot
proliferation
Rooting
MACRONUTRIENTS
KNO3 1800 1800 900
NH4NO3 400 400 200
Ca(NO3)·4H2O 1199.46 1199.46 599.73
CaCl2 ·2H2O – – –
MgSO4 · 7H2O 359.65 359.65 179.825
KH2PO4 270 270 135
K2SO4 MICRONUTRIENTS
– – –
H3BO3 6.20 4.80 4.80
CoCl2·6H2O 0.025 – –
CuSO4·5H2O 0.025 0.25 0.25
MnSO4·H2O 0.76 33.50 33.50
Na2MoO4·2H2O 0.25 0.39 0.39
KI 0.08 – –
ZnSO4·7H2O 8.60 17.00 17.00
FeSO4·7H2O 27.80 33.80 33.80
Na-EDTA·2H2O VITAMINS
37.30 45.40 45.40
Thiamine 1 2 2
Nicotinic acid 1 1 1
Biotin 0.1 – –
Folic acid 0.01 – –
p-amino benzoic acid 1 – –
Riboflavin 0.1 – –
Ca-pantothenate 0.5 – –
ORGANICS
– –
Myo-Inositol 50 100 100
Glycine – 2 2
Disinfection and node dissection. Shoots are taken from rapidly growing new
branches during spring (Figure 3A). Expanded leaves are removed. Shoots are
washed with water and detergent (Mistol®, Henkel Ibérica, S.A.). The protocol for
disinfection with ethanol and bleach is followed accordingly as described earlier.
Node explants, 2 cm long, are cut and cultured upright with the basal end of the
node embedded a few mm into the culture medium (Figure 3B).