Protocols for Micropropagation of Woody Trees and Fruits

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J.M. AL-KHAYRI
3. Water the transplants with 100 mg l –1 N-P-K fertilizer (20-20-20) and keep
potted plantlets in clear plastic enclosures under culture room conditions.
Gradually reduce humidity by adjusting air flow in the plastic enclosures
(Figure 4E). Upon noting any signs of welting, mist with water and readjust
opening as necessary.
4. After 1 month transfer plantlets to a cool shaded greenhouse for further
growth, after 3 to 6 months transfer to a shade house and maintain for 12 to
24 months depending on cultivar, and then transplant in the field (Figure 5
A–C).
Figure 4. Rooting and complete date palm plantlets. A) Shoot development and root
initiation. B) Shoot and root elongation, from left to right, with 0.2 mg l –1 NAA, no hormones,
and 0.2 mg l –1 IBA, respectively. C) Complete plantlets after rinsing agar residues. D) Soil
transfer of plantlets. E) Plantlets in an acclimatization chamber as an alternative to plastic
bags or mist systems.