Protocols for Micropropagation of Woody Trees and Fruits

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vi TABLE OF CONTENTS 14. Root induction of Pinus sylvestris L. hypocotyl cuttings using specific ectomycorrhizal fungi in vitro…………….....……..147 K. Niemi and C. Scagel 15. Micropropagation of Betula pendula Roth including genetically modified material……………………………….....…..153 H. Häggman, S. Sutela and M. Welander 16. Protocol for doubled-haploid micropropagation in Quercus suber L. and assisted verification………….....………163 B. Pintos, J.A. Manzanera and M.A. Bueno 17. In vitro propagation of Fraxinus species………………………….179 J.W. Van Sambeek and J.E. Preece 18. Micropropagation of black locust (Robinia pseudoacacia L.)…...193 J. Zhang, Y. Liu and H. Wang 19. Albizia odoratissima L.F. (Benth) micropropagation…………….201 V. Rajeswari and K. Paliwal 20. Micropropagation of Salix caprea L. ……………………………..213 G. Naujoks 21. Micropropagation of Cedrela fissilis Vell. (Meliaceae)..................221 E.C. Nunes, W.L.S. Laudano, F.N. Moreno, C.V. Castilho, P. Mioto, F.L. Sampaio, J.H. Bortoluzi, E.E. Benson, M.G. Pizolatti, E. Carasek and A.M. Viana 22. Micropropagation of mature trees of Ulmus glabra, Ulmus minor and Ulmus laevis…………………………….....……237 J. Malá, M. Cvikrová and V. Chalupa Section B 23. Micrografting in grapevine (Vitis spp.)…………………………...249 M. Mhatre and V.A. Bapat 24. Micrografting grapevine for virus indexing…………………...…259 R. Pathirana and M. Mckenzie 25. Apricot micropropagation…………………………………………267 O. Pérez-Tornero and L. Burgos 26. In vitro conservation and micropropagation of breadfruit (Artocarpus altilis, Moracea)……………....……….279 S.J. Murch, D. Ragone, W.L. Shi, A.R. Alan and P.K. Saxena 27. Micrografting of pistachio (Pistacia vera L. cv. Siirt)……………289 A. Onay, E. Tilkat, C. Isikalan and S. Namli 28. Protocol for micropropagation of Castanea sativa……………….299 A.M. Vieitez, M.C. Sánchez, M.L. García-Nimo and A. Ballester
29. Micropropagation of cashew (Anacardium occidentale L.)……...313 Thimmappaiah, R.A. Shirly and R.D. Iyer 30. In vitro mutagenesis and mutant multiplication………………….323 S. Predieri and N. Di Virgilio 31. In vitro propagation of nutmeg, Myristica fragrans Houtt………335 R.I. Iyer 32. TABLE OF CONTENTS Micropropagation of elite neem tree (Azadirachta indica A. Juss.)……………………………………………….....….345 B.K. Biswas and S.C. Gupta 33. Micropropagation protocol for microspore embryogenesis in Olea europaea L. ………………………….....…361 B. Pintos, A. Martin and M.A. Bueno 34. Micropropagation of Prunus domestica and Prunus salicina using mature seeds...............................................................373 L. Tian and S.I. Sibbald 35. Micropropagation of Juglans regia L. ............................................381 D. Ríos Leal, M. Sánchez-Olate, F. Avilés, M.E. Materan, M. Uribe, R. Hasbún. and R. Rodríguez 36. Tissue culture propagation of Mongolian cherry (Prunus fruticosa L.) and Nanking cherry (Prunus tomentosa L.)..............391 K. Pruski 37. Micropropagation of fig tree (Ficus carica sp)...............................409 M. Pasqual and E.A. Ferreira 38. High frequency shoot formation of yellow passion fruit (Passiflora edulis F. flavicarpa) via thin cell layer (TCL) technology..........................................................................................417 D.T. Nhut, B.L.T. Khiet, N.N. Thi, D.T.T. Thuy, N. Duy, N.T. Hai and P.X. Huyen 39. Micropropagation of Calabash tree Crescentia cujete L. .............427 C. Liu, S. He, R. Romero, S.J. Murch and P.K. Saxena 40. Micropropagation of papaya (Carica papaya L.)...........................437 M. Mishra, N. Shukla and R. Chandra Section C 41. Protocol for micropropagation of selected Vaccinium spp. ........445 M.G. Ostrolucká, A. Gajdošová, G. Libiaková, K. Hrubíková and M. Bežo 42. Protocol for micropropagation of Vaccinium vitis-idaea L. .........457 A. Gajdošová, M.G. Ostrolucká, G. Libiaková and E. Ondrušková vii
Page 2 and 3: PROTOCOLS FOR MICROPROPAGATION OF W
Page 4 and 5: A C.I.P. Catalogue record for this
Page 8 and 9: viii TABLE OF CONTENTS 43. Micropro
Page 10 and 11: x PREFACE on precise stepwise proto
Page 12 and 13: CHAPTER 1 TOTIPOTENCY AND THE CELL
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Page 24 and 25: CHAPTER 2 MICROPROPAGATION VIA ORGA
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Page 28 and 29: MICROPROPAGATION OF SLASH PINE Amon
Page 30 and 31: 2.6. Field Testing MICROPROPAGATION
Page 32 and 33: CHAPTER 3 MICROPROPAGATION OF COAST
Page 34 and 35: MICROPROPAGATION OF SEQUOIA SEMPERV
Page 42 and 43: CHAPTER 4 MICROPROPAGATION OF PINUS
Page 44 and 45: MICROPROPAGATION OF PINUS PINEA L.
Page 50 and 51: 42 2.1. Organ Culture K. ISHII ET A
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48 Table 3. Effects of plant growth
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50 K. ISHII ET AL. Gupta, P.K. & Du
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52 C. HARGREAVES AND M. MENZIES (Me
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54 C. HARGREAVES AND M. MENZIES Con
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56 2.1.3. Organogenesis from Field-
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58 C. HARGREAVES AND M. MENZIES Fig
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60 C. HARGREAVES AND M. MENZIES Fig
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62 C. HARGREAVES AND M. MENZIES For
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64 C. HARGREAVES AND M. MENZIES and
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CHAPTER 7 GENETIC FIDELITY ANALYSES
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PLANT GENETIC FIDELITY 69 Plant mat
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e added to samples, as this fluoroc
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11. Determine the mean channel numb
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3. In addition to testing various b
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GeneScan internal size standards: 4
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3500 3000 2500 2000 1500 1000 500 0
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PLANT GENETIC FIDELITY 81 microprop
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PLANT GENETIC FIDELITY 83 Steinkell
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86 2.1. Explant Preparation M.G. OS
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88 WPM (Lloyd & McCown, 1980) Macro
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90 M.G. OSTROLUCKÁ ET AL. on the m
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CHAPTER 9 MICROPROPAGATION OF MEDIT
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2.1. Explant Preparation MICROPROPA
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MICROPROPAGATION OF MEDITERRANEAN C
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108 D.T. NHUT ET AL. Larue (1953) a
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110 D.T. NHUT ET AL. HgCl2 added wi
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112 2.4. Establishment of Shoot Cul
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114 D.T. NHUT ET AL. Table 5. Effec
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116 D.T. NHUT ET AL. culture to be
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118 D. EWALD ability of the plant m
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120 D. EWALD Figure 1. Yew micropro
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122 D. EWALD Root development. Afte
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CHAPTER 12 MICROPROPAGATION OF LARI
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MICROPROPAGATION OF LARIX SPECIES V
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CHAPTER 13 PROPAGATION OF SELECTED
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PROPAGATION OF SELECTED PINUS GENOT
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CHAPTER 14 ROOT INDUCTION OF PINUS
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ROOT INDUCTION OF PINUS SYLVESTRIS
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ROOT INDUCTION OF PINUS SYLVESTRIS
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CHAPTER 15 MICROPROPAGATION OF BETU
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MICROPROPAGATION OF BETULA PENDULA
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CHAPTER 16 PROTOCOL FOR DOUBLED-HAP
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DOUBLED-HAPLOID MICROPROPAGATION IN
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CHAPTER 17 IN VITRO PROPAGATION OF
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IN VITRO PROPAGATION OF FRAXINUS SP
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Axillary shoots (number) IN VITRO P
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CHAPTER 18 MICROPROPAGATION OF BLAC
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MICROPROPAGATION OF BLACK LOCUST 19
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2.5. Rooting and Acclimatization MI
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MICROPROPAGATION OF BLACK LOCUST 19
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202 V. RAJESWARI AND K. PALIWAL tha
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204 V. RAJESWARI AND K. PALIWAL Mak
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206 V. RAJESWARI AND K. PALIWAL 2.3
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208 V. RAJESWARI AND K. PALIWAL Fig
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210 2.8. Hardening V. RAJESWARI AND
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CHAPTER 20 MICROPROPAGATION OF SALI
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MICROPROPAGATION OF SALIX CAPREA L.
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CHAPTER 21 MICROPROPAGATION OF CEDR
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2.1. Establishment of Shoot Culture
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MICROPROPAGATION OF CEDRELA FISSILI
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238 programmes is somatic embryogen
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240 Figure 2. A) Induction of organ
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242 2.4.1. Rooting of Apical and Ba
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244 of donor individuals and/or by
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246 J. MALÀ ET AL. Karnosky, D.F.,
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CHAPTER 23 MICROGRAFTING IN GRAPEVI
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MICROGRAFTING IN GRAPEVINE 251 and
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MICROGRAFTING IN GRAPEVINE 253 Tabl
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2.4. Culture Conditions MICROGRAFTI
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MICROGRAFTING IN GRAPEVINE 257 Feuc
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CHAPTER 24 MICROGRAFTING GRAPEVINE
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MICROGRAFTING GRAPEVINE FOR VIRUS I
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CHAPTER 25 APRICOT MICROPROPAGATION
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APRICOT MICROPROPAGATION Figure 1.
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APRICOT MICROPROPAGATION Figure 2.
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APRICOT MICROPROPAGATION 2.2.2. Hyp
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2.4. Acclimatization APRICOT MICROP
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APRICOT MICROPROPAGATION occurs fre
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CHAPTER 26 IN VITRO CONSERVATION AN
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IN VITRO CONSERVATION AND MICROPROP
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CHAPTER 27 MICROGRAFTING OF PISTACH
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MICROGRAFTING OF PISTACHIO Constitu
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MICROGRAFTING OF PISTACHIO 2.2.2. C
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MICROGRAFTING OF PISTACHIO 6. Incub
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MICROGRAFTING OF PISTACHIO 9. The r
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CHAPTER 28 PROTOCOL FOR MICROPROPAG
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MICROPROPAGATION OF CASTANEA SATIVA
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CHAPTER 29 MICROPROPAGATION OF CASH
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MICROPROPAGATION OF CASHEW 2.2.1. E
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MICROPROPAGATION OF CASHEW axillary
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MICROPROPAGATION OF CASHEW Table 1.
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MICROPROPAGATION OF CASHEW shade an
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CHAPTER 30 IN VITRO MUTAGENESIS AND
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IN VITRO MUTAGENESIS AND MUTANT MUL
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336 R.I. IYER compounds (Iyer et al
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A 338 2.2. Culture Medium R.I. IYER
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340 R.I. IYER Figure 2. Formation o
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342 R.I. IYER Figure 3. Formation o
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344 R.I. IYER Rao, Y.S., Mathew, K.
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346 B.K. BISWAS AND S.C. GUPTA marg
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348 B.K. BISWAS AND S.C. GUPTA (iv)
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350 B.K. BISWAS AND S.C. GUPTA 2.4.
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352 B.K. BISWAS AND S.C. GUPTA Figu
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354 B.K. BISWAS AND S.C. GUPTA tree
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356 B.K. BISWAS AND S.C. GUPTA Figu
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358 B.K. BISWAS AND S.C. GUPTA Drew
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CHAPTER 33 MICROPROPAGATION PROTOCO
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MICROPROPAGATION FOR MICROSPORE EMB
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374 L. TIAN AND S.I. SIBBALD younge
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376 L. TIAN AND S.I. SIBBALD Table
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378 L. TIAN AND S.I. SIBBALD 2.4. R
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CHAPTER 35 MICROPROPAGATION OF JUGL
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MICROPROPAGATION OF JUGLANS REGIA L
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CHAPTER 36 TISSUE CULTURE PROPAGATI
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PROPAGATION OF MONGOLIAN CHERRY AND
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410 M. PASQUAL AND E.A. FERREIRA 2.
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414 M. PASQUAL AND E.A. FERREIRA ch
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418 D.T. NHUT ET AL. its economical
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420 D.T. NHUT ET AL. Table 1. Shoot
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422 D.T. NHUT ET AL. Figure 3. Orga
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424 D.T. NHUT ET AL. mg l -1 NAA +
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426 D.T. NHUT ET AL. Nakasone, H.Y.
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428 C. LIU ET AL. C. cujete L. is c
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430 C. LIU ET AL. Table 1. Composit
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432 C. LIU ET AL. 6. Excise the sho
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434 Fresh weight per plantlet (mg)
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436 C. LIU ET AL. 4. REFERENCES Aga
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438 M. MISHRA ET AL. micropropagati
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440 M. MISHRA ET AL. each plate ino
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Section C
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446 M.G. OSTROLUCKÁ ET AL. from me
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448 M.G. OSTROLUCKÁ ET AL. regener
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450 M.G. OSTROLUCKÁ ET AL. Figure
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452 M.G. OSTROLUCKÁ ET AL. 2.6.3.
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454 counts counts M.G. OSTROLUCKÁ
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CHAPTER 42 PROTOCOL FOR MICROPROPAG
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AN medium (Anderson, 1980) MICROPRO
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MICROPROPAGATION OF VACCINIUM VITIS
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2.6. Flow Cytometry Analysis MICROP
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CHAPTER 43 MICROPROPAGATION OF BAMB
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BAMBOO MICROPROGATION BY AXILLARY S
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CHAPTER 44 IN VITRO CULTURE OF TREE
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IN VITRO CULTURE OF TREE PEONY 479
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500 M. MHATRE from various natural
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502 2.2. Disinfection of Plant Mate
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504 M. MHATRE soil in polythene bag
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506 M. MHATRE Figure 2. Pineapple,
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508 M. MHATRE Escalona, M., Lorenzo
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510 J.M. AL-KHAYRI Tunisia, Morocco
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512 J.M. AL-KHAYRI 2.1.2. Separatio
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514 2.2. Culture Medium J.M. AL-KHA
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516 J.M. AL-KHAYRI 5. Isolate callu
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518 J.M. AL-KHAYRI 3. Water the tra
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520 J.M. AL-KHAYRI expressed from c
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522 J.M. AL-KHAYRI alcohol and twic
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524 J.M. AL-KHAYRI potential. Simil
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526 J.M. AL-KHAYRI Barreveld, W.H.
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528 D.T. NHUT ET AL. arsenide chip
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530 D.T. NHUT ET AL. Figure 2. Plan
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532 D.T. NHUT ET AL. Figure 4. Plan
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534 D.T. NHUT ET AL. plantlets. The
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536 D.T. NHUT ET AL. Figure 11. Fre
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538 D.T. NHUT ET AL. Figure 13. Sub
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540 D.T. NHUT ET AL. The response o
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CHAPTER 48 IN VITRO MUTAGENESIS IN
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IN VITRO MUTAGENESIS IN BANANA 545
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3.3. Observations to be Recorded IN
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