Protocols for Micropropagation of Woody Trees and Fruits

PROPAGATION OF MONGOLIAN CHERRY AND NANKING CHERRY
Table 3. TDZ concentration effect on shoot number and shoot length in Mongolian Cherry
and Nanking Cherry (Pruski et al., 2005).
Species Treatment
TDZ (µM)
Mongolian
cherry
Nanking
cherry
Average number
of shoots
Average length
of shoots (mm)
0.00 2.1 26.1
0.45 4.2 20.6
0.90 4.5 15.4
1.80 5.0 13.7
0.00 1.4 26.6
0.45 2.1 23.5
0.90 3.2 18.4
1.80 4.5 15.4
2.2.6. Rooting
Root differentiation is stimulated by auxins that usually are applied to the base of the
shoots. Root induction may take place in different ways, the most often IBA is
included in the rooting medium and the cytokinin is omitted. Also, when rooting
in vitro, the concentration of salts in rooting media is reduced significantly, often by
50%. Figure 2D presents in vitro rooted plantlet of black fruiting Nanking cherry.
Similar to other fruit-bearing woody species (Maene & Debergh, 1983; Pruski
et al., 1991; Harada & Murai, 1996; Pruski et al., 2000), in vitro produced shoot
rosettes of both Mongolian and Nanking cherry produce roots readily ex vitro when
they are treated with auxins. On average, shoots of Mongolian cherry are somewhat
easier to root (average 73% rooting) than shoots of Nanking cherry (63%).
Treatment with auxin(s) definitely enhances rooting percentage compared to
untreated controls. Dai et al. (2001) reported effectiveness of IBA application for
rooting of Mongolian cherry. The best rooting has been observed when IBA/NAA
combination is used (Table 4). In our experiments, rooting was performed in two
ways, in vitro and ex vitro. The rooting ex vitro method was superior to the in vitro
system. The peat moss/perlite (1:1 v/v) mixture was packed in Hillson-type plastic
rootrainers with 32 cells per tray and one in vitro derived shoot was placed in each
cavity. Trays were kept on the bottom heated (25 o C) greenhouse bench equipped
with an intermittent misting system. The auxin treatments were introduced as daily
watering for the first week of the rooting period. A commercial rooting powder,
Rootone F containing 0.057%IBA/0.067%NAA mixture, was applied as a basal dip
on microcuttings prior to planting.
Rootone F proved to be almost equally effective as IBA/NAA combination.
Mongolian and Nanking cherry plantlets, rooted on the greenhouse bench under mist,
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