Protocols for Micropropagation of Woody Trees and Fruits

DOUBLED-HAPLOID MICROPROPAGATION IN QUERCUS SUBER L.
Tree
allele
(bp)
Figure 9. Microsatellite (SSR) composition of the parent tree showing heterozygous genotype
and of embryo samples, showing only one allele per locus. Allele sizes in bp. Number of antherinduced
embryos bearing each allele (SsrQpZAG15, SsrQpZAG46, SsrQpZAG110) detected
in cork oak tree. * 1:1 segregation at the 0.05 significance level (chi-squared test).
4. REFERENCES
Bueno, M.A., Astorga, R. & Manzanera, J.A. (1992a) Plant regeneration through somatic embryogenesis in
Quercus suber. Physiol. Plant. 85, 30–34.
Bueno, M.A. & Manzanera, J.A. (1992b) Primeros ensayos de inducción de embriones somáticos de Quercus
suber L. Scientia gerundensis. 18, 29–37.
Bueno, M.A., Gómez, A., Boscaiu, M., Manzanera, J.A. & Vicente, O. (1997) Stress-induced formation of
haploid plants through anther culture in cork oak (Quercus suber). Physiol. Plant. 99, 335–341.
Bueno, M.A. & Manzanera, J.A. (2003) Oak anther culture. In Maluszynski, M., Kasha, K.J., Foster, B.P.
& Szarejko, I. (Eds). Doubled Haploid Production in Crops Plants. A Manual. Kluwer Academic
Publishers, Dordrecht. ISBN 1-4020-1544-5, pp. 297–301.
120
124
Tree
allele
(bp)
190
192
Tree
allele
(bp)
222
238
ANTHER
A B C D
---
6
6
---
---
6
1
5
ANTHER
A B C D
---
6
4
2
---
6
6
---
ANTHER
A B C D
6
---
---
6
6
---
6
---
177
Total No.
embryos
7
17
Total No.
embryos
10*
14*
Total No.
embryos
18
6