Protocols for Micropropagation of Woody Trees and Fruits

470 V.M. JIMÉNEZ AND E. GUEVARA
Table 3. PGR combinations that produced highest bud break in different bamboo species.
Compound(s) Concentration Species Reference
BAP +
kinetin
2.5 mg l –1 +
1 mg l –1 Bambusa balcooa Das & Pal (2005b)
BAP + 2 mg l
kinetin +
coconut water
–1 +
1 mg l –1 + Bambusa tulda Das & Pal (2005a)
8%
BAP 1 mg l –1 Bambusa ventricosa Huang & Huang (1995)
BAP 2 mg l –1 Bambusa vulgaris Ramanayake et al. (2006)
BAP + 2 mg l
kinetin
–1 +
0.1 mg l –1
Dendrocalamus
Ramanayake &
giganteus
Yakandawala (1997)
BAP + 2.7 mg l
kinetin
–1 +
0.65 mg l –1
Dendrocalamus
Saxena & Bhojwani
longispathus
(1993)
BAP + 0.2 mg l
kinetin +
coconut water
–1 +
0.5 mg l –1 Dendrocalamus strictus
+ Bambusa arundinacea Nadgir et al. (1984)
10%
Bambusa vulgaris
BAP 1 mg l –1 Guadua angustifolia Marulanda et al. (2002)
BAP 3 mg l –1 Guadua angustifolia Jiménez et al. (2006)
Release of brown-colored exudates (probably phenolic compounds) seems to be a
problem during establishment of bamboo nodal explants in certain cases. More frequent
subcultures (every 1–2 weeks) generally suffice to avoid a detrimental effect
of these exudates. Liquid culture medium is helpful to alleviate these problems
(Das & Pal, 2005a,b).
To induce growth of lateral shoots at the base of the growing axis (Figure 3B),
transfer well-developed axes, about 6–8 cm long, to proliferation medium (Table 4)
contained in larger vessels. It is important that the developing shoots can grow without
contacting the vessel.
Sometimes the medium used for bud break also worked for shoot proliferation
(Saxena & Bhojwani, 1993; Das & Pal, 2005b).
Figure 3. Growth of the bamboo nodal segments. A) Developing axis, B) Development of
lateral shoots.