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Protocols for Micropropagation of Woody Trees and Fruits

Protocols for Micropropagation of Woody Trees and Fruits

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CHAPTER 9<br />

MICROPROPAGATION OF MEDITERRANEAN<br />

CYPRESS (CUPRESSUS SEMPERVIRENS L.)<br />

A. GIOVANELLI 1 AND A. DE CARLO 2<br />

1 IVALSA/Istituto per la Valorizzazione del Legno e delle Specie Arboree, Consiglio<br />

Nazionale delle Ricerche (CNR), 50010 Sesto Firentino (Firenze), Italy.<br />

E-mail: giovanelli@ivalsa.cnr.it<br />

2 IGV/Istituto di Genetica Vegetale, Sezione Firenze, Consiglio Nazionale della<br />

Ricerche (CNR), 50019 Sesto Fiorentino (Firenze), Italy.<br />

E-mail: decarlo@ivalsa.cnr.it<br />

1. INTRODUCTION<br />

The “Mediterranean” or “common” cypress (Cupressus sempervirens L.) is a tall<br />

tree (up to 30 m in height), belonging to the genus Cupressus, Family Cupressaceae.<br />

The Genus includes as much as 25 species (Ducrey et al., 1999), largely diffused in<br />

the Mediterranean basin, in Asia <strong>and</strong> in North America. Of them, the Mediterranean<br />

cypress is by far the most important <strong>and</strong> widespread species in the Mediterranean<br />

area. It is native to Northern Persia, as well as Syria, Turkey, Cyprus <strong>and</strong> several<br />

Greek isl<strong>and</strong>s. However, during the Roman Empire it was introduced into all the<br />

Mediterranean countries, where it can now be considered naturalised. The cypress is<br />

monoecious, <strong>and</strong> bears male <strong>and</strong> female strobili (cones) separately at the end <strong>of</strong><br />

short branchlets. Depending on the crown branch habit, the species is divided into<br />

two varieties, i.e.:<br />

– C. sempervirens var. horizontalis, the most common in natural areas, characterised<br />

by spreading branches <strong>and</strong> a broad conical crown;<br />

– C. sempervirens var. pyramidalis (= var. fastigiata), the most popular <strong>for</strong> ornamental<br />

use because <strong>of</strong> its erect branches, parallel to the trunk, which give the tree<br />

its typical columnar shape.<br />

The Mediterranean cypress plays fundamental ecological, economical <strong>and</strong> ornamental<br />

roles in the Mediterranean region. Indeed, the species has important characteristics<br />

<strong>of</strong> marked drought hardiness, <strong>and</strong> suitability in difficult terrains such as<br />

calcareous, clayey or rocky soils. As regards the cypress timber, it has interesting<br />

93<br />

S.M. Jain <strong>and</strong> H. Häggman (eds.), <strong>Protocols</strong> <strong>for</strong> <strong>Micropropagation</strong> <strong>of</strong> <strong>Woody</strong> <strong>Trees</strong> <strong>and</strong> <strong>Fruits</strong>, 93–105.<br />

© 2007 Springer.

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